Kang S G, Hannapel D J
Department of Horticulture, Iowa State University, Ames 50011, USA.
Plant Mol Biol. 1996 May;31(2):379-86. doi: 10.1007/BF00021798.
A potato MADS-box gene cDNA (POTM1-1) from an early tuber cDNA library has been isolated and characterized. The deduced amino acid sequence of POTM1-1 cDNA encodes 250 amino acids of a putative transcription factor containing a MADS-box domain and a K-box domain. These conserved domains share high homologies to those of flower-specific homeotic proteins, TM4 of tomato and AP1 of Arabidopsis, indicating that POTM1-1 gene is a homologue of the AP1 gene family. The levels of POTM1-1 transcripts were high in axillary buds, underground stolen tips, and newly formed tubers, but relatively low in mature tubers. During axillary bud development in a model petiole-leaf cutting system, the levels of POTM1-1 transcripts were abundant in actively growing shoots and during the early stages of microtuber development. It is possible that POTM1-1 functions as a transcription factor that regulates plant developmental processes in a number of tissue types.
从一个早期块茎cDNA文库中分离并鉴定了一个马铃薯MADS-box基因cDNA（POTM1-1）。POTM1-1 cDNA推导的氨基酸序列编码一个包含MADS-box结构域和K-box结构域的假定转录因子的250个氨基酸。这些保守结构域与花特异性同源异型蛋白、番茄的TM4和拟南芥的AP1的保守结构域具有高度同源性，表明POTM1-1基因是AP1基因家族的一个同源物。POTM1-1转录本在腋芽、地下匍匐茎顶端和新形成的块茎中水平较高，但在成熟块茎中相对较低。在一个模型叶柄-叶片扦插系统的腋芽发育过程中，POTM1-1转录本在活跃生长的芽和微型块茎发育早期水平丰富。POTM1-1有可能作为一种转录因子，在多种组织类型中调节植物发育过程。
