Li Z, Deutscher M P
Department of Biochemistry University of Connecticut Health Center Farmington 06030, USA.
Cell. 1996 Aug 9;86(3):503-12. doi: 10.1016/s0092-8674(00)80123-3.
tRNA maturation consists of the specific removal of precursor sequences from both the 5' and 3' termini of an initial RNA transcript. How this is accomplished has heretofore not been ascertained in any system. Using Northern analysis of RNA isolated from a variety of RNase-deficient E. coli strains, we have identified the processing intermediates that accumulate in the absence of specific processing nucleases. From this information we have established the maturation pathways for 12 different E. coli tRNAs including the specific role of each of the relevant RNases in the process. The surprising conclusion from this work is that tRNA maturation is a stochastic process that lacks a defined order and that can proceed with a variety of alternative 3' processing nucleases.
转运RNA（tRNA）成熟过程包括从初始RNA转录本的5'和3'末端特异性去除前体序列。迄今为止，在任何系统中都尚未确定这一过程是如何完成的。通过对从多种核糖核酸酶缺陷型大肠杆菌菌株中分离出的RNA进行Northern分析，我们鉴定出了在缺乏特定加工核酸酶时积累的加工中间体。根据这些信息，我们建立了12种不同大肠杆菌tRNA的成熟途径，包括每种相关核糖核酸酶在该过程中的具体作用。这项工作得出的惊人结论是，tRNA成熟是一个随机过程，缺乏确定的顺序，并且可以由多种替代的3'加工核酸酶进行。
