Ellis J A, Jackson M L, Bartsch R C, McGill L G, Martin K M, Trask B R, Haines D M
Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.
J Am Vet Med Assoc. 1996 Aug 15;209(4):767-71.
To determine whether there was intralesional infection or expression of FeLV or feline sarcoma virus in suspected vaccine-associated fibrosarcomas in cats.
Prospective case series.
130 suspected vaccine-associated fibrosarcomas from cats and 1 multicentric fibrosarcoma from 1 cat.
Excisional biopsy specimens were fixed in formalin and embedded in paraffin. Expression of FeLV antigen was assessed, using a polyclonal goat anti-FeLV glycoprotein 70 (gp 70) serum and an avidinbiotin immunoperoxidase staining technique. The FeLV genome was detected with a polymerase chain reaction (PCR), using primers targeted to a conserved sequence in the untranslated region of the long terminal repeat (LTR) of the FeLV.
FeLV gp 70 and LTR sequence were detected in a multicentric fibrosarcoma. All 130 of the suspected vaccine-associated fibrosarcomas were FeLV gp 70 negative on the basis of immunohistochemical test results: 100 fibrosarcomas also were examined by use of PCR and were negative for FeLV LTR region.
Exogenous retroviruses, FeLV, and feline sarcoma virus were not detected in these suspected vaccine-associated fibrosarcomas, using immunohistochemistry and PCR. Additional testing will be required to determine the nature of genomic alterations that are involved in the oncogenesis of vaccine-associated fibrosarcomas in cats.
确定猫疑似疫苗相关纤维肉瘤内是否存在病灶内感染或猫白血病病毒（FeLV）或猫肉瘤病毒的表达。
前瞻性病例系列研究。
130例来自猫的疑似疫苗相关纤维肉瘤以及1例来自1只猫的多中心纤维肉瘤。
切除活检标本用福尔马林固定并石蜡包埋。使用多克隆山羊抗FeLV糖蛋白70（gp 70）血清和抗生物素蛋白-生物素免疫过氧化物酶染色技术评估FeLV抗原的表达。使用针对FeLV长末端重复序列（LTR）非翻译区保守序列的引物，通过聚合酶链反应（PCR）检测FeLV基因组。
在一多中心纤维肉瘤中检测到FeLV gp 70和LTR序列。根据免疫组织化学检测结果，所有130例疑似疫苗相关纤维肉瘤的FeLV gp 70均为阴性：100例纤维肉瘤也通过PCR检测，FeLV LTR区域为阴性。
使用免疫组织化学和PCR在这些疑似疫苗相关纤维肉瘤中未检测到外源性逆转录病毒、FeLV和猫肉瘤病毒。需要进一步检测以确定参与猫疫苗相关纤维肉瘤肿瘤发生的基因组改变的性质。
