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通过协同凝集反应检测来自包虫囊肿液体样本中的包虫抗原。

Detection of hydatid antigen by co-agglutination in fluid samples from hydatid cysts.

作者信息

Parija S C, Ravinder P T, Shariff M

机构信息

Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, Pondicherry, India.

出版信息

Trans R Soc Trop Med Hyg. 1996 May-Jun;90(3):255-6. doi: 10.1016/s0035-9203(96)90237-0.

DOI:10.1016/s0035-9203(96)90237-0
PMID:8758068
Abstract

The co-agglutination (Co-A) procedure was standardized and evaluated to detect hydatid antigen in fluid samples aspirated from hydatid cysts. Samples were collected from 14 hydatid cysts in different organs (liver, 7; lung, 5; multiple abdominal cysts, 1; and kidney, 1) by surgery. Echinococcus granulosus scolices and hooklets were seen in only 6 (42.85%) samples by routine microscopy. In contrast, hydatid antigen was demonstrated in all the samples by Co-A (100% sensitivity). Co-A could be employed as a simple and rapid diagnostic procedure, as an alternative to microscopy, to confirm the hydatid aetiology of a suspected cyst.

摘要

对协同凝集(Co-A)程序进行了标准化和评估,以检测从包虫囊肿抽吸的液体样本中的包虫抗原。通过手术从不同器官(肝脏,7例;肺,5例;多发性腹部囊肿,1例;肾脏,1例)的14个包虫囊肿中采集样本。通过常规显微镜检查,仅在6个(42.85%)样本中发现了细粒棘球绦虫头节和小钩。相比之下,Co-A在所有样本中均检测到包虫抗原(敏感性为100%)。Co-A可作为一种简单快速的诊断程序,替代显微镜检查,以确认疑似囊肿的包虫病因。

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2
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引用本文的文献

1
Urinary hydatid antigen detection by coagglutination, a cost-effective and rapid test for diagnosis of cystic echinococcosis in a rural or field setting.通过协同凝集试验检测尿液棘球蚴抗原,这是一种在农村或野外环境中诊断囊性棘球蚴病的经济高效且快速的检测方法。
J Clin Microbiol. 2000 Aug;38(8):2972-4. doi: 10.1128/JCM.38.8.2972-2974.2000.
2
Detection of hydatid antigen in urine by countercurrent immunoelectrophoresis.用对流免疫电泳法检测尿液中的棘球蚴抗原。
J Clin Microbiol. 1997 Jun;35(6):1571-4. doi: 10.1128/jcm.35.6.1571-1574.1997.