Zhang G, Aldridge S, Clarke M C, McCauley J W
Institute for Animal Health, Compton Laboratory, Newbury, UK.
J Gen Virol. 1996 Aug;77 ( Pt 8):1677-81. doi: 10.1099/0022-1317-77-8-1677.
Cells infected with two closely related isolates of bovine viral diarrhoea virus (BVDV), one cytopathic (CP) and one non-cytopathic (NCP), were examined for signs of apoptosis. The results from labelling DNA using terminal transferase and biotinylated dUTP and by observing oligonucleosomal-sized DNA fragmentation indicated that the CP strain of BVDV induced apoptosis in cell culture but the NCP strain did not. Induction of apoptosis correlated with infected cells undergoing apoptosis rather than interactions between infected and uninfected cells and the induction of apoptosis by CP BVDV was a dominant trait in cells co-infected with both types of virus.
对感染了两种密切相关的牛病毒性腹泻病毒（BVDV）分离株的细胞进行了凋亡迹象检查，其中一种是细胞病变型（CP），另一种是非细胞病变型（NCP）。使用末端转移酶和生物素化dUTP标记DNA以及观察寡核小体大小的DNA片段化的结果表明，BVDV的CP株在细胞培养中诱导凋亡，而NCP株则不诱导。凋亡的诱导与正在经历凋亡的感染细胞相关，而不是与感染细胞和未感染细胞之间的相互作用相关，并且CP BVDV诱导的凋亡是同时感染两种病毒类型的细胞中的显性特征。
