RNA聚合酶II的转录终止子与酵母复制起点相关。
Transcriptional terminators of RNA polymerase II are associated with yeast replication origins.
作者信息
Chen S, Reger R, Miller C, Hyman L E
机构信息
Department of Biochemistry, SL-43, School of Medicine, Tulane University, New Orleans, LA 70112, USA.
出版信息
Nucleic Acids Res. 1996 Aug 1;24(15):2885-93. doi: 10.1093/nar/24.15.2885.
Abstract
The compact organization of the Saccharomyces cerevisiae genome necessitates that non-coding regulatory sequences reside in close proximity to one another. Here we show there is an intimate association between transcription terminators and DNA replication origins. Four replication origins were analyzed in a reporter gene assay that detects sequences that direct 3' end formation of mRNA transcripts. All four replication origins function as orientation-independent transcription terminators in this system, producing truncated polyadenylated mRNAs. Despite this close association, the cis-acting elements that confer replication origin function are genetically separable from those required for transcription termination. Several models are explored in an attempt to address how and why the signals specifying transcription termination and replication initiation overlap.
摘要
酿酒酵母基因组的紧密组织使得非编码调控序列彼此相邻。在这里，我们表明转录终止子与DNA复制起点之间存在密切关联。在一项报告基因检测中分析了四个复制起点，该检测可检测指导mRNA转录本3'末端形成的序列。在这个系统中，所有四个复制起点都作为不依赖方向的转录终止子发挥作用，产生截短的多聚腺苷酸化mRNA。尽管存在这种紧密关联，但赋予复制起点功能的顺式作用元件在遗传上可与转录终止所需的元件分离。探讨了几种模型，试图解决指定转录终止和复制起始的信号如何以及为何重叠的问题。
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