Chrisey L A, Lee G U, O'Ferrall C E
Naval Research Laboratory, Washington, DC 20375-5348, USA.
Nucleic Acids Res. 1996 Aug 1;24(15):3031-9. doi: 10.1093/nar/24.15.3031.
The covalent attachment of thiol-modified DNA oligomers; to self-assembled monolayer silane films on fused silica and oxidized silicon substrates is described. A heterobifunctional crosslinking molecule bearing both thiol- and amino-reactive moieties was used to tether a DNA oligomer (modified at its terminus with a thiol group) to an aminosilane film formed on silica surfaces. A variety of aminosilanes, crosslinkers and treatment conditions have been tested to identify optimal conditions for DNA immobilization using this approach. The DNA films which result have been characterized using UV spectroscopy, water contact angle measurement, radiolabeling and hybridization methods.
本文描述了硫醇修饰的DNA寡聚物与熔凝石英和氧化硅衬底上自组装单层硅烷膜的共价连接。使用一种带有硫醇和氨基反应性基团的异双功能交联分子，将一个在末端用硫醇基团修饰的DNA寡聚物连接到在二氧化硅表面形成的氨基硅烷膜上。已经测试了多种氨基硅烷、交联剂和处理条件，以确定使用这种方法固定DNA的最佳条件。所得到的DNA膜已通过紫外光谱、水接触角测量、放射性标记和杂交方法进行了表征。
