San Millan R, Ezkurra P A, Quindós G, Robert R, Senet J M, Pontón J
Departamento de Inmunología, Facultad de Medicina y Odontología, Universidad del País Vasco, Vizcaya, Spain.
Microbiology (Reading). 1996 Aug;142 ( Pt 8):2271-7. doi: 10.1099/13500872-142-8-2271.
The adhesion of Candida albicans to polystyrene and the effect of three monoclonal antibodies (mAbs) reactive with C. albicans cell wall surface antigens on this process was assessed in vitro with several C. albicans strains. In the absence of mAbs, adhesion of C. albicans to polystyrene increased in parallel with germ-tube formation. However, the growth of the strains in the yeast phase at 25 degrees C or the use of an agerminative mutant inhibited adhesion to polystyrene. Serotype A and B strains showed similar kinetics of adhesion to polystyrene and no statistically significant differences in germination or adhesion were observed when strains from the two serotypes were compared. The three mAbs had different effects on both germination and adhesion of C. albicans. mAbs 3D9 showed no influence on either germination or adhesion to polystyrene in two C. albicans strains. mAb B9E decreased both adhesion (45.6%) and filamentation (52.6%), and mAb 21E6 decreased filamentation (34.0%) but enhanced adhesion by 23.3%. This enhancement was also observed with the agerminative mutant and it was dose-dependent. It was not related to the binding capacity of the MAb to polystyrene nor to an increase in cell surface hydrophobicity of the antibody-treated cells. In conclusion, both growth phases of C. albicans can adhere to polystyrene, although the conditions for this process seem to be different in each phase. The two types of adhesion of C. albicans to polystyrene might have a role in the colonization of medical implants. The disparate effects shown by mAbs directed against cell wall mannoproteins of C. albicans on the adhesion of the fungus to polystyrene should be taken into consideration when designing strategies to block the adhesion of C. albicans to plastic materials with mAbs.
在体外对几种白色念珠菌菌株评估了白色念珠菌对聚苯乙烯的黏附以及三种与白色念珠菌细胞壁表面抗原反应的单克隆抗体（mAb）在此过程中的作用。在没有单克隆抗体的情况下，白色念珠菌对聚苯乙烯的黏附与芽管形成平行增加。然而，菌株在25℃酵母期的生长或使用无芽管形成突变体均抑制了对聚苯乙烯的黏附。A血清型和B血清型菌株对聚苯乙烯的黏附动力学相似，当比较两种血清型的菌株时，在发芽或黏附方面未观察到统计学上的显著差异。这三种单克隆抗体对白色念珠菌的发芽和黏附具有不同影响。单克隆抗体3D9对两种白色念珠菌菌株的发芽或对聚苯乙烯的黏附均无影响。单克隆抗体B9E使黏附（45.6%）和丝状化（52.6%）均降低，而单克隆抗体21E6使丝状化（34.0%）降低，但使黏附增加了23.3%。在无芽管形成突变体中也观察到这种增加，并且它是剂量依赖性的。这与单克隆抗体对聚苯乙烯的结合能力无关，也与抗体处理细胞的细胞表面疏水性增加无关。总之，白色念珠菌的两个生长阶段均可黏附于聚苯乙烯，尽管此过程在每个阶段的条件似乎不同。白色念珠菌对聚苯乙烯的两种黏附类型可能在医用植入物的定植中起作用。在设计用单克隆抗体阻断白色念珠菌对塑料材料黏附的策略时，应考虑针对白色念珠菌细胞壁甘露糖蛋白的单克隆抗体对真菌黏附于聚苯乙烯所表现出的不同影响。
