人视网膜内皮细胞中葡萄糖转运蛋白（GLUT - 1和GLUT - 3）的调控
Regulation of glucose transporters (GLUT-1 and GLUT-3) in human retinal endothelial cells.
作者信息
Knott R M, Robertson M, Muckersie E, Forrester J V
出版信息
Biochem J. 1996 Aug 15;318 ( Pt 1)(Pt 1):313-7. doi: 10.1042/bj3180313.
Abstract
The regulation of glucose transporters (GLUT-1 and GLUT-3), in terms of both mRNA and protein, in human retinal endothelial cells was investigated. The cells responded within 1 h of exposure to 5 mM glucose with an increase in the level of GLUT-3 mRNA that was due to an increase in the transcription of the 4.1 kb mRNA of the gene for GLUT-3. In the absence of glucose, the gene for GLUT-1 was not transcribed but the level of GLUT-3 mRNA was increased in these conditions and this was the result of an increase in the transcription of the 4.1 kb mRNA. The level of GLUT-1 and GLUT-3 mRNA was maximal when the cells were exposed to 15 mM glucose. These results are discussed in the light of the glucose regulatory potential of the retinal microvasculature and the implications that this may have for the mechanisms of diabetic retinopathy.
摘要
研究了人视网膜内皮细胞中葡萄糖转运蛋白（GLUT-1和GLUT-3）在mRNA和蛋白质水平上的调节情况。细胞在暴露于5 mM葡萄糖1小时内做出反应，GLUT-3 mRNA水平增加，这是由于GLUT-3基因4.1 kb mRNA转录增加所致。在无葡萄糖的情况下，GLUT-1基因不转录，但此时GLUT-3 mRNA水平增加，这是4.1 kb mRNA转录增加的结果。当细胞暴露于15 mM葡萄糖时，GLUT-1和GLUT-3 mRNA水平达到最大值。根据视网膜微血管系统的葡萄糖调节潜力以及这可能对糖尿病视网膜病变机制产生的影响对这些结果进行了讨论。
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