蛋白磷酸酶2A的Aα亚基的结晶
Crystallization of the A alpha subunit of protein phosphatase 2A.
作者信息
Skidmore M O, Sawaya M R, Parkin S, Rupp B, Hope H, Everse S J, Walter G
机构信息
Department of Pathology, University of California at San Diego, La Jolla 92093-0612, USA.
出版信息
Protein Sci. 1996 Jun;5(6):1198-200. doi: 10.1002/pro.5560050625.
Abstract
The A alpha subunit of human protein phosphatase 2A forms crystals in space group P2(1) with cell dimensions a = 104.0, b = 174.9, c = 168.2 A, and beta angle = 90.2 degrees. At cryogenic temperatures, the crystals diffracted to a resolution limit of approximately 3.0 A. Based on the unit cell dimensions and a calculated molecular mass of 65,277 Da, the Matthews coefficient suggests eight molecules per asymmetric unit. Two native data sets were collected to a nominal resolution of 3.0 A and merged to provide a set that is 93% complete, with Rsym of 9.9%.
摘要
人蛋白磷酸酶2A的Aα亚基在空间群P2(1)中形成晶体，晶胞参数为a = 104.0 Å，b = 174.9 Å，c = 168.2 Å，β角 = 90.2°。在低温下，晶体衍射分辨率极限约为3.0 Å。根据晶胞尺寸和计算得到的分子量65277 Da，马修斯系数表明每个不对称单位中有八个分子。收集了两个天然数据集，名义分辨率为3.0 Å，并合并以提供一个完整度为93%、Rsym为9.9%的数据集。
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本文引用的文献
1
Protein phosphatase 2A--a 'ménage à trois'.蛋白磷酸酶2A——一种“三人组合”。
Trends Cell Biol. 1994 Aug;4(8):287-91. doi: 10.1016/0962-8924(94)90219-4.
2
Role of protein phosphatase 2A in Drosophila development.
Semin Cancer Biol. 1995 Aug;6(4):249-56. doi: 10.1006/scbi.1995.0032.
3
Regulation by tumour antigens defines a role for PP2A in signal transduction.肿瘤抗原介导的调控作用明确了PP2A在信号转导中的作用。
Semin Cancer Biol. 1995 Aug;6(4):229-37. doi: 10.1006/scbi.1995.0030.
4
Protein serine/threonine phosphatases: structure, regulation, and functions in cell growth.蛋白质丝氨酸/苏氨酸磷酸酶：细胞生长中的结构、调节及功能
Physiol Rev. 1993 Oct;73(4):673-99. doi: 10.1152/physrev.1993.73.4.673.
5
Protein serine/threonine phosphatases and cell transformation.蛋白质丝氨酸/苏氨酸磷酸酶与细胞转化
Biochim Biophys Acta. 1993 Aug 23;1155(2):207-26. doi: 10.1016/0304-419x(93)90005-w.
6
Molecular model of the A subunit of protein phosphatase 2A: interaction with other subunits and tumor antigens.蛋白磷酸酶2A A亚基的分子模型：与其他亚基及肿瘤抗原的相互作用
J Virol. 1994 Jan;68(1):123-9. doi: 10.1128/JVI.68.1.123-129.1994.
7
Solvent content of protein crystals.蛋白质晶体的溶剂含量。
J Mol Biol. 1968 Apr 28;33(2):491-7. doi: 10.1016/0022-2836(68)90205-2.
8
Cryocrystallography of ribosomal particles.核糖体颗粒的低温晶体学
Acta Crystallogr B. 1989 Apr 1;45 ( Pt 2):190-9. doi: 10.1107/s0108768188013710.
9
Molecular cloning and sequence of cDNA encoding polyoma medium tumor antigen-associated 61-kDa protein.编码多瘤病毒中肿瘤抗原相关61千道尔顿蛋白的cDNA的分子克隆及序列分析
Proc Natl Acad Sci U S A. 1989 Nov;86(22):8669-72. doi: 10.1073/pnas.86.22.8669.
10
Crystallography of biological macromolecules at ultra-low temperature.生物大分子在超低温下的晶体学
Annu Rev Biophys Biophys Chem. 1990;19:107-26. doi: 10.1146/annurev.bb.19.060190.000543.
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