Skidmore M O, Sawaya M R, Parkin S, Rupp B, Hope H, Everse S J, Walter G
Department of Pathology, University of California at San Diego, La Jolla 92093-0612, USA.
Protein Sci. 1996 Jun;5(6):1198-200. doi: 10.1002/pro.5560050625.
The A alpha subunit of human protein phosphatase 2A forms crystals in space group P2(1) with cell dimensions a = 104.0, b = 174.9, c = 168.2 A, and beta angle = 90.2 degrees. At cryogenic temperatures, the crystals diffracted to a resolution limit of approximately 3.0 A. Based on the unit cell dimensions and a calculated molecular mass of 65,277 Da, the Matthews coefficient suggests eight molecules per asymmetric unit. Two native data sets were collected to a nominal resolution of 3.0 A and merged to provide a set that is 93% complete, with Rsym of 9.9%.
人蛋白磷酸酶2A的Aα亚基在空间群P2(1)中形成晶体，晶胞参数为a = 104.0 Å，b = 174.9 Å，c = 168.2 Å，β角 = 90.2°。在低温下，晶体衍射分辨率极限约为3.0 Å。根据晶胞尺寸和计算得到的分子量65277 Da，马修斯系数表明每个不对称单位中有八个分子。收集了两个天然数据集，名义分辨率为3.0 Å，并合并以提供一个完整度为93%、Rsym为9.9%的数据集。
