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[超薄切片在肾脏疾病尸检形态学诊断中的应用]

[Ultrathin sections in postmortem morphological diagnosis of kidney diseases].

作者信息

Engelhardt J, Mágori A, Ormos J

出版信息

Morphol Igazsagugyi Orv Sz. 1977 Apr;17(2):90-8.

PMID:876264
Abstract

Authors for the light-microscopic examination of various--mainly glomerular--diseases of kidneys in addition to paraffin section have used also ultrathin sections (0,5 mu). Material was fixed in buffered-formalin solution and embedded in metacrylate or Durcupan ACM. Sections obtained by this method can be studied by electron micro-scope as well. Sections were stained with methylene-blue and basicfuchsine, further with periodic-acid silver methenamine. The use of ultrathin sections for the light-microscopic studies can not replace the study of paraffin-sections. Ultrathin sections are suitable for the detection of fine details. They can be studied by light- and electron microscopy as well.

摘要

除石蜡切片外,对各种(主要是肾小球)肾脏疾病进行光学显微镜检查的作者们也使用了超薄切片(0.5微米)。材料用缓冲福尔马林溶液固定,并包埋在甲基丙烯酸酯或Durcupan ACM中。通过这种方法获得的切片也可以用电子显微镜进行研究。切片用亚甲蓝和碱性品红染色,进一步用高碘酸银甲胺染色。将超薄切片用于光学显微镜研究并不能取代石蜡切片的研究。超薄切片适用于检测细微细节。它们也可以用光学显微镜和电子显微镜进行研究。

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