Engelhardt J, Mágori A, Ormos J
Morphol Igazsagugyi Orv Sz. 1977 Apr;17(2):90-8.
Authors for the light-microscopic examination of various--mainly glomerular--diseases of kidneys in addition to paraffin section have used also ultrathin sections (0,5 mu). Material was fixed in buffered-formalin solution and embedded in metacrylate or Durcupan ACM. Sections obtained by this method can be studied by electron micro-scope as well. Sections were stained with methylene-blue and basicfuchsine, further with periodic-acid silver methenamine. The use of ultrathin sections for the light-microscopic studies can not replace the study of paraffin-sections. Ultrathin sections are suitable for the detection of fine details. They can be studied by light- and electron microscopy as well.
除石蜡切片外，对各种（主要是肾小球）肾脏疾病进行光学显微镜检查的作者们也使用了超薄切片（0.5微米）。材料用缓冲福尔马林溶液固定，并包埋在甲基丙烯酸酯或Durcupan ACM中。通过这种方法获得的切片也可以用电子显微镜进行研究。切片用亚甲蓝和碱性品红染色，进一步用高碘酸银甲胺染色。将超薄切片用于光学显微镜研究并不能取代石蜡切片的研究。超薄切片适用于检测细微细节。它们也可以用光学显微镜和电子显微镜进行研究。
