Ormos J, Engelhardt J, Mágori A
Acta Morphol Acad Sci Hung. 1977;25(1):61-70.
For the light microscopic postmortem study of mostly glomerular renal diseases, in addition to the paraffin technique, 0.5 mu thick (semithin) sections from material fixed in buffered formaldehyde and embedded in methacrylate or Durcupan ACM were used. The method allows for eventual electron microscopic examinations. The semithin sections were stained with methylene blue combined with basic fuchsin, as well as with periodic acid-silver methamine. The method is not a substitution, but the supplementation of the paraffin technique and is suited for the clarification of numerous fine details: in some cases the exact diagnosis was made in this way.
对于大多数肾小球性肾脏疾病的光学显微镜下尸检研究，除石蜡技术外，还使用了取自用缓冲甲醛固定并包埋在甲基丙烯酸酯或Durcupan ACM中的材料制成的0.5微米厚（半薄）切片。该方法允许进行最终的电子显微镜检查。半薄切片用亚甲蓝结合碱性品红以及过碘酸-银甲胺染色。该方法不是石蜡技术的替代方法，而是其补充方法，适用于阐明许多细微细节：在某些情况下，通过这种方式做出了准确的诊断。
