Izaki S, Yamamoto T, Goto Y, Ishimaru S, Yudate F, Kitamura K, Matsuzaki M
Department of Dermatology, Saitama Medical School, Japan.
Br J Dermatol. 1996 Jun;134(6):1060-4.
Platelet-activating factor (PAF), as well as PAF acetylhydrolase (PAF-AH) activity in the peripheral blood plasma of patients with psoriasis and palmoplantar pustolosis, was measured with a radioimmunoassay technique, and compared with leukotriene (LT) B4, LTC4, LTD4 and E4 (LTD4/E4), thromboxane (TX) B2 and prostaglandin (PG) E2 levels. In a normal healthy group (n = 12) PAF level was 25.9 +/- 6.5 pg/0.1 ml plasma (mean +/- standard error of the mean: SEM), and this was elevated in patients with psoriasis (68.1 +/- 11.8, n = 25, P < 0.01), without a change in the PAF-AH level. LTB4 showed a similar increase (115.0 +/- 21.6 pg/ml vs. 68.2 +/- 11.8 pg/ml, P < 0.05), while TXB2 and PGE2 showed insignificant (P > 0.05) changes. LTC4 and LTD4/E4 were around the level of the limit of detection. Patients with palmoplantar pustulosis (n = 33) demonstrated similar, but milder and statistically insignificant, increases in PAF, LTB4, TXB2 and PGE2 levels. Modulation of the mediator levels before and after treatment was compared in 16 patients with psoriasis and 11 with palmoplantar pustulosis. PAF in psoriasis significantly decreased after treatment (70.9 +/- 17.1 to 25.1 +/- 5.5, P < 0.05) and this was moderately correlated (r = 0.298) with clinical improvement as indicated by the psoriasis area and severity index (38.5 +/- 7.5 to 10.9 +/- 4.2, P < 0.01). TXB2 (180.2 +/- 100.4 to 34.1 +/- 13.5), PGE2 (3.7 +/- 0.7 to 2.9 +/- 0.5) and LTB4 (120.1 +/- 31.1 to 84.2 +/- 8.2), in psoriasis, mildly decreased without statistical significance. Patients with palmoplantar pustulosis demonstrated a similar decrease in all mediators without statistical significance. The results obtained suggest a role of PAF in psoriasis. As the priming effects of PAF have been shown, for leucocytes and endothelial cells, to enhance their inflammatory response, we assume that PAF has roles in the acute phase of psoriatic and leucotactic inflammation.
采用放射免疫测定技术检测银屑病和掌跖脓疱病患者外周血浆中的血小板活化因子（PAF）以及PAF乙酰水解酶（PAF-AH）活性，并与白三烯（LT）B4、LTC4、LTD4和E4（LTD4/E4）、血栓素（TX）B2和前列腺素（PG）E2水平进行比较。在正常健康组（n = 12）中，PAF水平为25.9±6.5 pg/0.1 ml血浆（平均值±平均标准误差：SEM），在银屑病患者中升高（68.1±11.8，n = 25，P < 0.01），而PAF-AH水平无变化。LTB4也有类似升高（115.0±21.6 pg/ml对68.2±11.8 pg/ml，P < 0.05），而TXB2和PGE2变化不显著（P > 0.05）。LTC4和LTD4/E4处于检测限水平左右。掌跖脓疱病患者（n = 33）的PAF、LTB4、TXB2和PGE2水平有类似但较轻微且无统计学意义的升高。比较了16例银屑病患者和11例掌跖脓疱病患者治疗前后介质水平的变化。银屑病患者治疗后PAF显著降低（70.9±17.1降至25.1±5.5，P < 0.05），且与银屑病面积和严重程度指数所表明的临床改善呈中度相关（r = 0.298）（38.5±7.5降至10.9±4.2，P < 0.01）。银屑病患者的TXB2（180.2±100.4降至34.1±13.5）、PGE2（3.7±0.7降至2.9±0.5）和LTB4（120.1±31.1降至84.2±8.2）轻度降低，但无统计学意义。掌跖脓疱病患者所有介质均有类似降低，但无统计学意义。所得结果提示PAF在银屑病中起作用。由于已表明PAF对白细胞和内皮细胞具有启动作用，可增强其炎症反应，我们推测PAF在银屑病急性期和白细胞趋化性炎症中起作用。
