Dhar A, Dockery P, O W S, Turner K, Lenton E A, Cooke I D
Department of Anatomy, University of Hong Kong.
J Anat. 1996 Jun;188 ( Pt 3)(Pt 3):671-6.
The human ovarian granulosa cell is perhaps the most widely studied endocrine cell, but little quantitative structural information exists for this cell. In the present study new and traditional stereological probes have been employed to provide quantitative structural information on these functionally important cells. Granulosa cells were obtained from follicular aspirations from 10 women during in vitro fertilisation procedures. Initially 2 methods were used to estimate the mean nuclear volume of these cells: the mean number weighted nuclear volume was estimated by the Selector and the mean volume weighted nuclear volume by the point sampled intercept method. It was found that the difference between the 2 volume estimates was only 8.5%. The volume weighted mean nuclear volume was used as an estimate of nuclear volume. This was subsequently corrected (taking the percentage difference as the empirical bias) and combined with fractional cell volumes (Vv) to produce estimates of cell, mitochondrial, lipid and nucleolar volume. The proportion of the cell occupied by the nucleus had a remarkably low interindividual variation (CV = 7.6%). The proportion of the nucleus occupied by euchromatin also had a striking low variation (CV < 6%). All other cellular parameters had CVs of less than 35%. The lipid composition of these cells showed the greatest interindividual variability, with a CV of 42% for relative and 54% for absolute volume. The present study outlines a simple protocol for the quantitation of granulosa cell structure using new unbiased stereological probes and providing baseline structural information.
人类卵巢颗粒细胞可能是研究最为广泛的内分泌细胞，但关于这种细胞的定量结构信息却很少。在本研究中，采用了新的和传统的体视学探针来提供关于这些功能重要细胞的定量结构信息。颗粒细胞取自10名女性在体外受精过程中的卵泡抽吸物。最初使用两种方法来估计这些细胞的平均核体积：通过Selector估计平均数量加权核体积，通过点抽样截距法估计平均体积加权核体积。结果发现，这两种体积估计值之间的差异仅为8.5%。体积加权平均核体积被用作核体积的估计值。随后对其进行了校正（将百分比差异作为经验偏差），并与细胞分数体积（Vv）相结合，以得出细胞、线粒体、脂质和核仁体积的估计值。细胞核所占细胞的比例个体间变异非常低（CV = 7.6%）。常染色质所占细胞核的比例变异也非常低（CV < 6%）。所有其他细胞参数的CV均小于35%。这些细胞的脂质组成个体间变异性最大，相对体积的CV为42%，绝对体积的CV为54%。本研究概述了一种使用新的无偏体视学探针定量颗粒细胞结构并提供基线结构信息的简单方案。
