多细胞原核生物黄色黏球菌中的酪氨酸磷酸化
Tyrosine phosphorylation in Myxococcus xanthus, a multicellular prokaryote.
作者信息
Frasch S C, Dworkin M
机构信息
Department of Microbiology, University of Minnesota, Minneapolis, 55455, USA.
出版信息
J Bacteriol. 1996 Jul;178(14):4084-8. doi: 10.1128/jb.178.14.4084-4088.1996.
Abstract
Tyrosine phosphorylation is an extremely rare event in prokaryotes, occurring almost exclusively in multicellular eukaryotes. We have identified, for the first time, by the use of antiphosphotyrosine monoclonal antibody and Western blot (immunoblot) analysis, two tyrosine-phosphorylated membrane proteins in the multicellular prokaryote Myxococcus xanthus. The pattern of tyrosine phosphorylation was shown to change during development, indicating a possible role for this regulatory modification during two stages of development, i.e., aggregation and sporulation. Furthermore, the altered pattern of tyrosine phosphorylation observed in a variety of signaling mutants was shown to differ from that observed in the wild type, suggesting further the possible involvement of tyrosine phosphorylation during the development program.
摘要
酪氨酸磷酸化在原核生物中是极其罕见的事件，几乎只发生在多细胞真核生物中。我们首次通过使用抗磷酸酪氨酸单克隆抗体和蛋白质免疫印迹（免疫印迹）分析，在多细胞原核生物黄色粘球菌中鉴定出两种酪氨酸磷酸化的膜蛋白。酪氨酸磷酸化模式在发育过程中发生变化，表明这种调节修饰在发育的两个阶段，即聚集和孢子形成过程中可能发挥作用。此外，在各种信号突变体中观察到的酪氨酸磷酸化模式的改变与野生型中观察到的不同，这进一步表明酪氨酸磷酸化可能参与发育程序。
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