Howe A Y, Tyrrell D L
Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Canada.
J Virol. 1996 Aug;70(8):5035-42. doi: 10.1128/JVI.70.8.5035-5042.1996.
Nucleocapsid assembly in hepadnavirus replication requires selective encapsidation of the pregenomic RNA template and the viral polymerase by the core proteins. It has been shown that an encapsidation signal located at the 5' end of the pregenomic RNA is responsible for its interaction with the polymerase. In the present study, we have shown that a region located at the 3' periphery of the core open reading frame may interact with the viral polymerase in duck hepatitis B virus. By using an in vitro rabbit reticulocyte lysate translation system, we found that interaction of the polymerase with this region resulted in selective suppression of core mRNA translation. Insertion of this putative inhibitory sequence into the CD4 gene also led to a selective inhibition of CD4 mRNA translation in the presence of polymerase. Specific inhibition of core protein synthesis was observed in a chicken hepatoma cell line (LMH) cotransfected with core and polymerase plasmid DNA.
嗜肝DNA病毒复制过程中的核衣壳组装需要核心蛋白对前基因组RNA模板和病毒聚合酶进行选择性包装。研究表明，位于前基因组RNA 5'端的一个包装信号负责其与聚合酶的相互作用。在本研究中，我们发现位于核心开放阅读框3'边缘的一个区域可能与鸭乙型肝炎病毒中的病毒聚合酶相互作用。通过使用体外兔网织红细胞裂解物翻译系统，我们发现聚合酶与该区域的相互作用导致核心mRNA翻译的选择性抑制。将这个假定的抑制序列插入CD4基因也会在有聚合酶存在的情况下导致CD4 mRNA翻译的选择性抑制。在用核心和聚合酶质粒DNA共转染的鸡肝癌细胞系（LMH）中观察到了核心蛋白合成的特异性抑制。
