Barker D E, Roizman B
Marjorie B. Kovler Viral Oncology Laboratories, University of Chicago, Illinois 60637.
J Virol. 1992 Jan;66(1):562-6. doi: 10.1128/JVI.66.1.562-566.1992.
We present evidence for the existence of an additional herpes simplex virus 1 gene designated UL49.5. The sequence, located between genes UL49 and UL50, predicts a hydrophobic protein with 91 amino acids. Attempts to delete UL49.5 were not successful. To demonstrate that UL49.5 is expressed, we made two recombinant viruses. First, we inserted in frame an oligonucleotide encoding a 15-amino-acid epitope known to react with a monoclonal antibody. This gene, consisting of the authentic promoter and chimeric coding domain, was inserted into the thymidine kinase gene of wild-type virus and in infected cells expressed a protein which reacted with the monoclonal antibody. The second recombinant virus contained a 5' UL49.5-thymidine kinase fusion gene. The protein expressed by this virus confirmed that the first methionine codon of UL49.5 served as the initiating codon. The predicted amino acid sequence of UL49.5 is consistent with the known properties of NC-7, a small capsid protein whose gene has not been previously mapped. A homolog of UL49.5 is present in the genome of varicella-zoster virus, located between homologs of UL49 and UL50.
我们提供了存在一种名为UL49.5的额外单纯疱疹病毒1型基因的证据。该序列位于基因UL49和UL50之间，预测为一种含有91个氨基酸的疏水蛋白。删除UL49.5的尝试未成功。为了证明UL49.5的表达，我们构建了两种重组病毒。首先，我们将一个编码已知可与单克隆抗体反应的15个氨基酸表位的寡核苷酸框内插入。这个由真实启动子和嵌合编码结构域组成的基因被插入野生型病毒的胸苷激酶基因中，并且在感染细胞中表达了一种可与单克隆抗体反应的蛋白质。第二种重组病毒包含一个5' UL49.5 - 胸苷激酶融合基因。该病毒表达的蛋白质证实了UL49.5的第一个甲硫氨酸密码子作为起始密码子。UL49.5的预测氨基酸序列与NC - 7的已知特性一致，NC - 7是一种小衣壳蛋白，其基因先前尚未定位。水痘带状疱疹病毒基因组中存在UL49.5的同源物，位于UL49和UL50的同源物之间。
