在感染重组杆状病毒的昆虫细胞中组装的单纯疱疹病毒衣壳：VP26的结构真实性和定位
Herpes simplex virus capsids assembled in insect cells infected with recombinant baculoviruses: structural authenticity and localization of VP26.
作者信息
Trus B L, Homa F L, Booy F P, Newcomb W W, Thomsen D R, Cheng N, Brown J C, Steven A C
机构信息
Laboratory of Structural Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892-2755, USA.
出版信息
J Virol. 1995 Nov;69(11):7362-6. doi: 10.1128/JVI.69.11.7362-7366.1995.
Abstract
Recently, recombinant baculoviruses have been used to show that expression of six herpes simplex virus type 1 genes results in the formation of capsid-like particles. We have applied cryoelectron microscopy and three-dimensional image reconstruction to establish their structural authenticity to a resolution of approximately 2.7 nm. By comparing capsids assembled with and without the expression of gene UL35, we have confirmed the presence of six copies of its product, VP26 (12 kDa), around each hexon tip. However, VP26 is not present on pentons, indicating that the conformational differences between the hexon and penton states of the major capsid protein, VP5, extend to the VP26 binding site.
摘要
最近，重组杆状病毒已被用于证明1型单纯疱疹病毒的六个基因的表达会导致衣壳样颗粒的形成。我们应用了冷冻电子显微镜和三维图像重建技术，将其结构真实性确定到约2.7纳米的分辨率。通过比较在有和没有基因UL35表达情况下组装的衣壳，我们证实了其产物VP26（12千道尔顿）的六个拷贝存在于每个六邻体顶端周围。然而，五邻体上不存在VP26，这表明主要衣壳蛋白VP5的六邻体和五邻体状态之间的构象差异延伸到了VP26结合位点。
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Assembly of herpes simplex virus type 1 capsids using a panel of recombinant baculoviruses.利用一组重组杆状病毒组装单纯疱疹病毒1型衣壳
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Assembly of herpes simplex virus (HSV) intermediate capsids in insect cells infected with recombinant baculoviruses expressing HSV capsid proteins.在感染了表达单纯疱疹病毒（HSV）衣壳蛋白的重组杆状病毒的昆虫细胞中组装HSV中间衣壳。
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