Trus B L, Homa F L, Booy F P, Newcomb W W, Thomsen D R, Cheng N, Brown J C, Steven A C
Laboratory of Structural Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892-2755, USA.
J Virol. 1995 Nov;69(11):7362-6. doi: 10.1128/JVI.69.11.7362-7366.1995.
Recently, recombinant baculoviruses have been used to show that expression of six herpes simplex virus type 1 genes results in the formation of capsid-like particles. We have applied cryoelectron microscopy and three-dimensional image reconstruction to establish their structural authenticity to a resolution of approximately 2.7 nm. By comparing capsids assembled with and without the expression of gene UL35, we have confirmed the presence of six copies of its product, VP26 (12 kDa), around each hexon tip. However, VP26 is not present on pentons, indicating that the conformational differences between the hexon and penton states of the major capsid protein, VP5, extend to the VP26 binding site.
最近，重组杆状病毒已被用于证明1型单纯疱疹病毒的六个基因的表达会导致衣壳样颗粒的形成。我们应用了冷冻电子显微镜和三维图像重建技术，将其结构真实性确定到约2.7纳米的分辨率。通过比较在有和没有基因UL35表达情况下组装的衣壳，我们证实了其产物VP26（12千道尔顿）的六个拷贝存在于每个六邻体顶端周围。然而，五邻体上不存在VP26，这表明主要衣壳蛋白VP5的六邻体和五邻体状态之间的构象差异延伸到了VP26结合位点。
