Tak P P, Hintzen R Q, Teunissen J J, Smeets T J, Daha M R, van Lier R A, Kluin P M, Meinders A E, Swaak A J, Breedveld F C
Department of General Internal Medicine, Leiden University Hospital, Leiden, The Netherlands.
Clin Immunol Immunopathol. 1996 Aug;80(2):129-38. doi: 10.1006/clin.1996.0106.
Differentiation of CD4+ T-cells is reflected by the change from the CD45RA+CD27+ phenotype via CD45RO+CD27+ to the CD45RO+CD27- phenotype. To provide insight into the migration and activation of T-cells at the site of inflammation in rheumatoid arthritis (RA), CD27 expression by T-cells in peripheral blood (PB), synovial fluid (SF), and synovial tissue (ST) as well as the levels of the soluble form of CD27 (sCD27) in plasma and SF were studied in patients with RA. Since CD4+CD27+ T-cells are involved in providing helper activity for B-cells, we also investigated the levels of rheumatoid factors in serum and SF in relation to CD27 expression. The mean level of sCD27, which is produced by CD27+ cells, and the mean percentage of CD27 T-cells within the CD4+CD45RA- subset were higher in SF than in PB. SF sCD27 levels were higher in the patients with RA than in the patients with osteoarthritis, who served as controls. In ST infiltration by CD4+CD45RO+CD27+ T-cells, could be demonstrated in the rheumatoid perivascular lymphocytic aggregates with a relative increase in the percentage of CD27- T-cells in the diffuse lymphocytic infiltrate. The sCD27 levels and the percentages of CD4+CD27+ cells in SF correlated positively with the levels of rheumatoid factors in serum and SF. The findings presented in this study suggest a continuous influx of preactivated CD4+CD45RO+CD27+ cells from the PB into the rheumatoid ST and further activation and differentiation to CD4+CD45RO+CD27- cells in situ, followed by migration to the SF. These activated T-cells are likely to play a role in synovial inflammation.
CD4+ T细胞的分化表现为从CD45RA+CD27+表型经CD45RO+CD27+转变为CD45RO+CD27-表型。为深入了解类风湿关节炎（RA）炎症部位T细胞的迁移和激活情况，对RA患者外周血（PB）、滑液（SF）和滑膜组织（ST）中T细胞的CD27表达以及血浆和SF中可溶性CD27（sCD27）水平进行了研究。由于CD4+CD27+ T细胞参与为B细胞提供辅助活性，我们还研究了血清和SF中类风湿因子水平与CD27表达的关系。由CD27+细胞产生的sCD27平均水平以及CD4+CD45RA-亚群内CD27+ T细胞的平均百分比在SF中高于PB。RA患者的SF sCD27水平高于作为对照的骨关节炎患者。在ST中，CD4+CD45RO+CD27+ T细胞浸润可在类风湿血管周围淋巴细胞聚集物中得到证实，在弥漫性淋巴细胞浸润中CD27- T细胞百分比相对增加。SF中的sCD27水平和CD4+CD27+细胞百分比与血清和SF中类风湿因子水平呈正相关。本研究结果表明，预激活的CD4+CD45RO+CD27+细胞持续从PB流入类风湿ST，并在原位进一步激活和分化为CD4+CD45RO+CD27-细胞，随后迁移至SF。这些活化的T细胞可能在滑膜炎症中起作用。
