Sun Huaqiang, Zhang Yeyong, Song Weiguo, Yin Luxu, Wang Gongteng, Yu Dejia, Zhang Qian, Yan Xinfeng, Li Shufeng
Department of Orthopedics, Shandong Qianfoshan Hospital, Shandong University, Jinan, Shandong, China.
Pharmacology Center, Shandong Academy of Chinese Medicine, Jinan, Shandong, China.
Hum Immunol. 2019 Apr;80(4):263-269. doi: 10.1016/j.humimm.2019.02.007. Epub 2019 Feb 12.
Synovial inflammation is observed in patients with osteoathritis (OA) and likely contributed to its exacerbation. Regulatory B (Breg) cells are shown to suppress inflammation in various diseases, including rheumatoid arthritis (RA). To examine whether Breg cells also participated in OA, we examined the synovial fluid from OA patients, and compared with that in RA patients. In OA synovial fluid, IL-10-producing B cells were present directly ex vivo and were increased upon stimulation, indicating that B cells were a source of IL-10 directly at the affected site of OA patients. Interestingly, the frequency of IL-10 cells in synovial B cells was higher in OA patients than in RA patients, but the total number of IL-10 B cells in OA was lower than that in RA, suggesting that OA patients presented lower B cell infiltration than RA patients. Phenotypical analysis demonstrated that the IL-10 B cells were IgM and CD27, but not CD24 or CD38. To allow functional analysis of IgMCD27 B cells, the IgMCD27 B cells in the blood of OA patients were examined. These blood IgMCD27 B cells expressed more IL-10, but less CD80 and CD86 than non-IgMCD27 B cells. Blood IgMCD27 B cells suppressed the proliferation and IFN-γ expression of autologous T cells, and this effect could be reverted if IL-10 was inhibited. Furthermore, we found that patients with more severe OA presented lower levels of IL-10 B cells in the synovial fluid. Together, our study described an IgMCD27 B cell subset in OA patients, which represented the major IL-10-secreting B cell type in the synovial fluid of OA patients and possessed regulatory function.
骨关节炎（OA）患者存在滑膜炎症，这可能促使病情加重。调节性B（Breg）细胞在包括类风湿关节炎（RA）在内的多种疾病中可抑制炎症。为研究Breg细胞是否也参与OA发病，我们检测了OA患者的滑液，并与RA患者的滑液进行比较。在OA滑液中，直接离体时即可发现产生白细胞介素10（IL-10）的B细胞，且刺激后数量增加，这表明B细胞是OA患者病变部位IL-10的直接来源。有趣的是，OA患者滑膜B细胞中IL-10细胞的频率高于RA患者，但OA中IL-10 B细胞的总数低于RA患者，这表明OA患者的B细胞浸润程度低于RA患者。表型分析显示，IL-10 B细胞为IgM和CD27阳性，但CD24或CD38阴性。为对IgMCD27 B细胞进行功能分析，我们检测了OA患者血液中的IgMCD27 B细胞。这些血液中的IgMCD27 B细胞比非IgMCD27 B细胞表达更多的IL-10，但CD80和CD86表达较少。血液中的IgMCD27 B细胞抑制自体T细胞的增殖和干扰素-γ（IFN-γ）表达，若抑制IL-10，这种作用可逆转。此外，我们发现OA病情越严重的患者，其滑液中IL-10 B细胞水平越低。总之，我们的研究描述了OA患者中的一个IgMCD27 B细胞亚群，它是OA患者滑液中主要的分泌IL-10的B细胞类型，并具有调节功能。
