Alizadeh P, Klionsky D J
Section of Microbiology, University of California, Davis 95616, USA.
FEBS Lett. 1996 Aug 12;391(3):273-8. doi: 10.1016/0014-5793(96)00751-x.
The disaccharide trehalose plays a critical role in yeast cell survival during conditions of environmental stress. The vacuole of the yeast Saccharomyces cerevisiae contains an enzyme, acid trehalase (ATH), that is capable of degrading trehalose. Recently, a gene required for ATH activity, ATH1, was cloned and sequenced [Destruelle et al., (1995) Yeast 11, 1015-1025]. The relationship between ATH1 and ATH, however, was not determined. We have purified ATH and shown that it is the ATH1 gene product; peptide sequences from the purified protein correspond to the deduced amino acid sequence of Ath1p. In addition, antiserum to Ath1p specifically recognizes purified ATH.
双糖海藻糖在酵母细胞应对环境压力时的存活过程中起着关键作用。酿酒酵母的液泡中含有一种能够降解海藻糖的酶，即酸性海藻糖酶（ATH）。最近，克隆并测序了ATH活性所需的基因ATH1[德斯特勒尔等人，（1995年）《酵母》11卷，第1015 - 1025页]。然而，ATH1与ATH之间的关系尚未确定。我们已经纯化了ATH，并证明它是ATH1基因的产物；纯化蛋白的肽序列与Ath1p推导的氨基酸序列相对应。此外，针对Ath1p的抗血清能特异性识别纯化的ATH。
