非洲爪蟾卵母细胞中与SRY相关的基因。

SRY-related genes in Xenopus oocytes.

作者信息

Miyata S, Miyashita K, Hosoyama Y

机构信息

Department of Chemistry, College of Humanities and Sciences, Nihon University, Tokyo, Japan.

出版信息

Biochim Biophys Acta. 1996 Jul 31;1308(1):23-7. doi: 10.1016/0167-4781(96)00091-7.

DOI:10.1016/0167-4781(96)00091-7

PMID:8765746

Abstract

SRY-related genes are known as Sox (Sry-box) genes. Two Sox cDNAs from Xenopus oocytes were analyzed. The deduced product of the Xenopus Sox gene (xSox-11) consisted of the standard domains of an HMG box, glycine/alanine-rich region and glutamic acid/aspartic acid-rich region and may be involved in the control of transcription. The other Sox gene (xSox-11-D) had a deletion of 262 nucleotides at one end of the HMG box in the xSox-11 cDNA. The deletion resulted in a frame-shift and in variations in base pair composition and the length of the trinucleotide repeat in the C-terminal coding region. The amino acid sequence of the C-terminal domain encoded by xSox-II-D was highly basic and might be involved, together with the HMG box, in the binding to DNA.

摘要

与SRY相关的基因被称为Sox（Sry盒）基因。对来自非洲爪蟾卵母细胞的两个Sox cDNA进行了分析。非洲爪蟾Sox基因（xSox - 11）推导的产物由一个HMG盒、富含甘氨酸/丙氨酸的区域和富含谷氨酸/天冬氨酸的区域等标准结构域组成，可能参与转录调控。另一个Sox基因（xSox - 11 - D）在xSox - 11 cDNA的HMG盒一端有262个核苷酸的缺失。该缺失导致了移码，并导致C端编码区碱基对组成和三核苷酸重复长度的变化。由xSox - II - D编码的C端结构域的氨基酸序列具有高度碱性，可能与HMG盒一起参与与DNA的结合。