一种人SRY相关cDNA hSOX20的分离与表达

Isolation and expression of a human SRY-related cDNA hSOX20.

作者信息

Hiraoka Y, Ogawa M, Sakai Y, Taniguchi K, Fujii T, Umezawa A, Hata J, Aiso S

机构信息

Department of Anatomy, Keio University School of Medicine, Tokyo, Japan.

出版信息

Biochim Biophys Acta. 1998 Mar 9;1396(2):132-7. doi: 10.1016/s0167-4781(97)00186-3.

DOI:10.1016/s0167-4781(97)00186-3

PMID:9540826

Abstract

SOX is a family of genes related to the testis-determining gene, SRY. We have isolated and sequenced an hSOX20 cDNA from a cell line of human embryonic carcinoma. This cDNA contains an open reading frame (ORF) encoding 233 amino acids. The protein encompasses an SRY-type HMG box exhibiting strong homologies to those of mouse Sox15 and Sox16. Various adult and fetal tissues were tested for hSOX20 mRNA by Northern analysis. Its expression is restricted to the fetal testis and the size of the transcript is 1.5 knt. Electrophoretic mobility shift assay indicated that recombinant hSOX20 polypeptide is capable of binding to AACAAT sequence.

摘要

SOX是一个与睾丸决定基因SRY相关的基因家族。我们从人胚胎癌细胞系中分离并测序了hSOX20 cDNA。该cDNA包含一个编码233个氨基酸的开放阅读框（ORF）。该蛋白质包含一个SRY型HMG框，与小鼠Sox15和Sox16的HMG框具有高度同源性。通过Northern分析检测了各种成人和胎儿组织中的hSOX20 mRNA。其表达仅限于胎儿睾丸，转录本大小为1.5 kb。电泳迁移率变动分析表明重组hSOX20多肽能够与AACAAT序列结合。