一种新型虹鳟鱼SRY型HMG盒蛋白rtSox23的cDNA克隆及其功能分析。
cDNA cloning of a novel rainbow trout SRY-type HMG box protein, rtSox23, and its functional analysis.
作者信息
Yamashita A, Suzuki S, Fujitani K, Kojima M, Kanda H, Ito M, Takamatsu N, Yamashita S, Shiba T
机构信息
Department of Biosciences, School of Science, Kitasato University, 1-15-1 Kitasato, Sagamihara, Kanagawa 228, Japan.
出版信息
Gene. 1998 Mar 16;209(1-2):193-200. doi: 10.1016/s0378-1119(98)00047-x.
We have isolated a cDNA clone for a new member of Sox genes, termed rtSox23, from a rainbow trout ovary cDNA library. rtSox23 mRNA was notably expressed in ovary and brain. rtSox23 contains a leucine zipper in addition to an SRY-type HMG box. Although the recombinant HMG box region protein of rtSox23 could bind to an AACAAT sequence, the full-length rtSox23 could form a homodimer and did not bind to the sequence. Furthermore, using a two-hybrid system, we have isolated a cDNA clone encoding a protein that bound to the leucine zipper region of rtSox23. This protein was the rainbow trout homologue of mouse nucleoporin p62, which is a component of the nuclear pore complex in nuclear envelope. The rainbow trout p62 mRNA was also prominent in ovary and brain. Taken together, these results suggest that the rainbow trout p62 associates with rtSox23 in vivo and modulates the function of rtSox23.
我们从虹鳟卵巢cDNA文库中分离出一个Sox基因新成员的cDNA克隆，命名为rtSox23。rtSox23 mRNA在卵巢和脑中显著表达。rtSox23除了含有一个SRY型HMG框外，还含有一个亮氨酸拉链。虽然rtSox23的重组HMG框区域蛋白能与AACAAT序列结合，但全长rtSox23能形成同源二聚体，且不与该序列结合。此外，利用双杂交系统，我们分离出一个编码与rtSox23亮氨酸拉链区域结合的蛋白的cDNA克隆。该蛋白是小鼠核孔蛋白p62的虹鳟同源物，核孔蛋白p62是核膜中核孔复合体的一个组成部分。虹鳟p62 mRNA在卵巢和脑中也很突出。综上所述，这些结果表明虹鳟p62在体内与rtSox23相互作用，并调节rtSox23的功能。
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