Nishi H
Analytical Research Laboratory, Tanabe Seiyaku Co., Ltd., Osaka, Japan.
J Chromatogr A. 1996 May 31;735(1-2):57-76. doi: 10.1016/0021-9673(95)01262-1.
This review surveys the enantiomer separation of drugs by electrokinetic chromatography (EKC). EKC is one option of capillary electrophoretic (CE) techniques, which permits the separation of electrically neutral drugs. In enantiomer separation by EKC, ionic pseudo-stationary phases such as chiral micelles and proteins, which can migrate with its electrophoretic mobility and can interact with the solutes, are employed as chiral selectors. Addition of electrically neutral chiral selectors such as cyclodextrins and precolumn conversion of solutes into diastereomers are also successful in EKC. A brief theory of separation and modes of EKC enantiomer separation are described with some typical applications.
本综述概述了采用电动色谱（EKC）对药物对映体进行分离的情况。EKC是毛细管电泳（CE）技术的一种，它能够分离电中性药物。在通过EKC进行对映体分离时，离子型假固定相，如手性胶束和蛋白质，可凭借其电泳迁移率进行迁移并与溶质相互作用，这些被用作手性选择剂。添加电中性手性选择剂，如环糊精，以及将溶质进行柱前转化为非对映体在EKC中也很成功。文中介绍了EKC对映体分离的简要分离理论和模式，并列举了一些典型应用。
