Stephen D W, Jamieson D J
Biomedical Research Centre, Ninewells Hospital and Medical School, University of Dundee, UK.
FEMS Microbiol Lett. 1996 Aug 1;141(2-3):207-12. doi: 10.1111/j.1574-6968.1996.tb08386.x.
The tripeptide gamma-L-glutamyl-L-cystinylglycine (glutathione) is one of the major antioxidant molecules of cells and is thought to play a vital role in buffering the cell against reactive oxygen species and toxic electrophiles. We wished to determine the role of glutathione in the protection of the yeast Saccharomyces cerevisiae against oxidative stress. This study shows that glutathione is an important antioxidant molecule in yeast, with gamma-glutamylcysteine synthetase (gsh1) mutants, deficient in glutathione synthesis, being hypersensitive to H2O2 and superoxide anions in both exponential- and stationary-phase cultures. Despite this, these mutants are still able to induce adaptive stress responses to oxidants.
三肽γ-L-谷氨酰-L-胱氨酰甘氨酸（谷胱甘肽）是细胞的主要抗氧化分子之一，被认为在缓冲细胞免受活性氧和有毒亲电试剂的侵害方面发挥着至关重要的作用。我们希望确定谷胱甘肽在保护酿酒酵母免受氧化应激中的作用。这项研究表明，谷胱甘肽是酵母中的一种重要抗氧化分子，缺乏谷胱甘肽合成的γ-谷氨酰半胱氨酸合成酶（gsh1）突变体在指数生长期和稳定期培养物中对过氧化氢和超氧阴离子都高度敏感。尽管如此，这些突变体仍然能够诱导对氧化剂的适应性应激反应。
