Delbaere A, Bergmann P J, Gervy-Decoster C, Deschodt-Lanckman M, de Maertelaer V, Englert Y
Department of Gynecology and Obstetrics, Erasme Hospital, Brussels, Belgium.
J Clin Endocrinol Metab. 1996 Aug;81(8):2810-5. doi: 10.1210/jcem.81.8.8768835.
Local variations in the ovarian renin-angiotensin system were investigated in peritoneal fluid (PF) during normal menstrual cycles and after ovarian stimulation with gonadotropins. PF was collected either during laparoscopy or by transvaginal aspiration before ovocyte retrieval for in vitro fertilization. Renin activity (RA) and angiotensin II immunoreactivity (Ang II-ir) were assayed in PF and simultaneously collected blood. The level of Ang II-ir was higher in PF than in plasma throughout the cycle, where as RA was in the same range of magnitude in the two compartments. In PF, Ang II-ir reached levels 2-5 times higher in the periovulatory period (days 12-14 and 15-17 of the cycle) than those found during the other stages of the cycle, whereas plasmatic Ang II-ir remained stable. No significant change could be demonstrated in RA throughout the cycle in either PF or plasma. Ovarian stimulation induced a strong elevation of Ang II-ir in PF, but not in plasma. High performance liquid chromatography revealed that the majority of Ang II-ir in PF was true Ang II. In conclusion, these results show a periovulatory elevation of Ang II in PF during the cycle and a more pronounced rise after treatment with gonadotropins. These observations support the involvement of Ang II in the process of ovulation or fecundation.
在正常月经周期以及用促性腺激素刺激卵巢后，对腹膜液（PF）中卵巢肾素 - 血管紧张素系统的局部变化进行了研究。PF是在腹腔镜检查期间或在体外受精取卵前经阴道抽吸收集的。对PF和同时采集的血液进行肾素活性（RA）和血管紧张素II免疫反应性（Ang II-ir）检测。在整个周期中，PF中Ang II-ir的水平高于血浆，而两个隔室中RA的水平在相同的数量范围内。在PF中，围排卵期（周期的第12 - 14天和第15 - 17天）Ang II-ir水平比周期其他阶段高出2 - 5倍，而血浆中Ang II-ir保持稳定。在PF或血浆中，整个周期RA均无明显变化。卵巢刺激导致PF中Ang II-ir显著升高，但血浆中未升高。高效液相色谱显示PF中大部分Ang II-ir是真正的Ang II。总之，这些结果表明周期中PF在围排卵期Ang II升高，用促性腺激素治疗后升高更明显。这些观察结果支持Ang II参与排卵或受精过程。
