Chun K T, Goebl M G
Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis 46202-5122, USA.
Genetics. 1996 Jan;142(1):39-50. doi: 10.1093/genetics/142.1.39.
The yeast Saccharomyces cerevisiae reproduces by budding, and many genes are required for proper bud development. Mutations in some of these genes cause cells to die with an unusual terminal morphology-elongated or otherwise aberrantly shaped buds. To gain insight into bud development, we set out to identify novel genes that encode proteins required for proper bud morphogenesis. Previous studies screened collections of conditional mutations to identify genes required for essential functions, including bud formation. However, genes that are not susceptible to the generation of mutations that cause a conditional phenotype will not be identified in such screens. To identify a more comprehensive collection of mutants, we used transposon mutagenesis to generate a large collection of lethal disruption mutations. This collection was used to identify 209 mutants with disruptions that cause an aberrant terminal bud morphology. The disruption mutations in 33 of these mutants identify three previously uncharacterized genes as essential, and the mutant phenotypes suggest roles for their products in bud morphogenesis.
酿酒酵母通过出芽进行繁殖，许多基因对于正常的芽发育是必需的。其中一些基因的突变会导致细胞以异常的终末形态死亡，即芽伸长或以其他异常形状出现。为了深入了解芽发育，我们着手鉴定编码正常芽形态发生所需蛋白质的新基因。先前的研究筛选了条件性突变体库，以鉴定包括芽形成在内的基本功能所需的基因。然而，在这种筛选中不会鉴定出不易产生导致条件性表型突变的基因。为了鉴定更全面的突变体集合，我们使用转座子诱变来产生大量致死性破坏突变。这个集合被用于鉴定209个具有导致异常终末芽形态破坏的突变体。其中33个突变体中的破坏突变确定了三个以前未被表征的基因为必需基因，并且突变体表型表明它们的产物在芽形态发生中发挥作用。
