Pitkäranta A, Hovi T, Karma P
Department of Otolaryngology, University of Helsinki, Finland.
Int J Pediatr Otorhinolaryngol. 1996 Jan;34(1-2):25-33. doi: 10.1016/0165-5876(95)01231-1.
We have previously shown that leukocyte cultures of children suffering from recurrent respiratory tract infections produce less interferon (IFN) than those of healthy children. In the present study this tentative marker of recurrent infections was used to study the pathogenetic background of otitis media with effusion (OME). Altogether 57 consecutive children, aged 2-11 years, who came for tympanostomy and/or adenoidectomy were divided into three subgroups: 25 of them had OME and a history of recurrent acute otitis media (rAOM/OME+), 20 had OME without an infectious background (inf-/OME+), and 12 had a history of recurrent upper respiratory infections (inf +/OME-) without OME. All the children were free of acute illness at the time of sampling. Differences between the groups were seen in IFN yields when leukocyte cultures were stimulated with adeno-, rhino-, corona-, respiratory syncytial or influenza A viruses. Leukocytes from inf-/OME+ children produced more IFN than those of the other two groups. Though no sex differences in the IFN responses were seen among rAOM/OME+ and inf +/OME- children, leukocytes from inf-/OME+ girls produced significantly higher amounts of IFN than those of inf-/OME+ boys, or rAOM/OME+ and inf +/OME- children. These differences between clinically different groups of children support the view that the etiology of OME can be heterogeneous.
我们之前已经表明，患有反复呼吸道感染的儿童的白细胞培养物产生的干扰素（IFN）比健康儿童的少。在本研究中，这种反复感染的初步标志物被用于研究分泌性中耳炎（OME）的发病机制背景。共有57名年龄在2至11岁之间、前来接受鼓膜切开术和/或腺样体切除术的连续儿童被分为三个亚组：其中25名患有OME且有反复急性中耳炎病史（rAOM/OME+），20名患有OME但无感染背景（inf-/OME+），12名有反复上呼吸道感染病史（inf +/OME-）但无OME。所有儿童在采样时均无急性疾病。当用腺病毒、鼻病毒、冠状病毒、呼吸道合胞病毒或甲型流感病毒刺激白细胞培养物时，各组之间在IFN产量上存在差异。inf-/OME+儿童的白细胞产生的IFN比其他两组更多。虽然在rAOM/OME+和inf +/OME-儿童中未观察到IFN反应的性别差异，但inf-/OME+女孩的白细胞产生的IFN量明显高于inf-/OME+男孩或rAOM/OME+和inf +/OME-儿童。临床上不同组儿童之间的这些差异支持了OME病因可能是异质性的观点。
