Daviss S R, Lewis D A
Department of Psychiatry, Western Psychiatric Institute und Clinic, University of Pittsburgh PA 15213, USA.
Psychiatry Res. 1995 Nov 29;59(1-2):81-96. doi: 10.1016/0165-1781(95)02720-3.
Schizophrenia has been reported to be associated with alterations in GABAergic local circuit neurons of the prefrontal cortex. In this study, immunocytochemical techniques and antibodies against the calcium-binding proteins calbindin (CB) and calretinin (CR) were used to determine the laminar distribution and relative density of separate subpopulations of local circuit neurons in prefrontal cortical areas 9 and 46 from five pairs of schizophrenic and control subjects, matched for age, sex, and post-mortem interval. The laminar distribution pattern of CB-immunoreactive local circuit neurons was similar in both schizophrenic and control subjects. In both prefrontal regions, however, the density of CB-labeled neurons was 50-70% greater in schizophrenic subjects compared with control subjects, with cortical layers III and V/VI being preferentially affected. In contrast, the density of CR-IR neurons did not differ significantly between schizophrenic and control subjects. These findings reveal a selective increase in the density of a subpopulation of GABAergic local circuit neurons in the prefrontal cortex. Although other explanations for these observations must be considered, they may be consistent with the hypothesis that gene expression in GABAergic neurons is altered in schizophrenia.
据报道，精神分裂症与前额叶皮质GABA能局部回路神经元的改变有关。在本研究中，运用免疫细胞化学技术以及针对钙结合蛋白钙结合蛋白（CB）和钙视网膜蛋白（CR）的抗体，来确定来自五对年龄、性别和死后间隔相匹配的精神分裂症患者和对照受试者的前额叶皮质9区和46区局部回路神经元不同亚群的分层分布和相对密度。CB免疫反应性局部回路神经元的分层分布模式在精神分裂症患者和对照受试者中相似。然而，在两个前额叶区域，与对照受试者相比，精神分裂症患者中CB标记神经元的密度要高出50 - 70%，其中皮质III层和V/VI层受到的影响尤为明显。相比之下，精神分裂症患者和对照受试者之间CR免疫反应性神经元的密度没有显著差异。这些发现揭示了前额叶皮质中GABA能局部回路神经元一个亚群的密度选择性增加。尽管必须考虑对这些观察结果的其他解释，但它们可能与精神分裂症中GABA能神经元基因表达发生改变的假说一致。
