Ghose U R, Parida B B
Zoology Department, Dhenkanal College, Dhenkanal, Orissa, India.
Indian J Cancer. 1995 Sep;32(3):95-9.
Buccal mucosa scrapings from 50 individuals belonging to tribes of Koraput district in Orissa State (India), were smeared and fixed. The cells were stained adopting Feulgen technique. All the tribes are active tobacco and alcohol users. The individuals were asked about their age, sex, food habit, tobacco and alcohol consumption habit, period of consumption and daily consumption quantity. Micronuclei were scored from the smeared cells as increase in micronucleus frequency in buccal mucosa cells of tobacco and alcohol users indicates a high risk group for oral cancer. At least 1000 cells per sample were screened. The frequency of micronucleated cells is found to be higher (7.37%) in case of male individuals than female individuals (5.90%). Individuals of both sexes of age group (50-65) years show higher frequency of micronucleus. Tobacco smokers with Pika habit show higher frequency of micronucleus (7.06%) than tobacco chewers with Dungia habit (6.33%). Such increase in micronucleus frequency in buccal mucosa cells indicates that the tribes are high risk of oral cancer.
对来自印度奥里萨邦科拉普特区部落的50个人的颊黏膜刮片进行涂片和固定。采用福尔根技术对细胞进行染色。所有部落的人都有吸烟和饮酒的习惯。询问了这些人的年龄、性别、饮食习惯、吸烟和饮酒习惯、消费时间和每日消费量。由于吸烟和饮酒者颊黏膜细胞中的微核频率增加表明其为口腔癌高危人群，因此对涂片细胞进行微核计数。每个样本至少筛选1000个细胞。发现男性个体的微核细胞频率（7.37%）高于女性个体（5.90%）。年龄在（50 - 65）岁的男女个体微核频率较高。有皮卡习惯的吸烟者微核频率（7.06%）高于有顿吉亚习惯的嚼烟者（6.33%）。颊黏膜细胞中微核频率的这种增加表明这些部落患口腔癌的风险很高。
