Matsumoto K
Second Department of Internal Medicine, Nihon University School of Medicine, Tokyo, Japan.
Nephron. 1996;73(2):305-9. doi: 10.1159/000189057.
The effect of recombinant human interleukin-10 (IL-10) on the in vitro cytokine secretion of peripheral blood monocytes (PBM) was evaluated in patients with IgA nephropathy (IgAN). Significantly increased spontaneous and lipopolysaccharide (LPS)-stimulated secretion of tumor necrosis factor-alpha and interleukin-8 was determined in PBM cultures of IgAN patients compared to those of normal controls. In the present study IL-10 inhibited the spontaneous as well as the LPS-stimulated cytokine secretion of PBM in IgAN. A significant inhibitory effect of IL-10 was observed in cultures of PBM from IgAN patients as well as from normal controls. When both LPS and anti-IL-10 antibody were added together to the PBM, a further increase in LPS-enhanced secretion of cytokines occurred. This study provides some novel information showing the inhibitory effects of IL-10 on cytokine secretion by PBM derived from IgAN patients.
在IgA肾病（IgAN）患者中评估了重组人白细胞介素-10（IL-10）对外周血单核细胞（PBM）体外细胞因子分泌的影响。与正常对照相比，IgAN患者的PBM培养物中肿瘤坏死因子-α和白细胞介素-8的自发分泌及脂多糖（LPS）刺激的分泌显著增加。在本研究中，IL-10抑制了IgAN患者PBM的自发及LPS刺激的细胞因子分泌。在来自IgAN患者以及正常对照的PBM培养物中均观察到IL-10的显著抑制作用。当将LPS和抗IL-10抗体一起添加到PBM中时，LPS增强的细胞因子分泌进一步增加。本研究提供了一些新信息，表明IL-10对IgAN患者来源的PBM细胞因子分泌具有抑制作用。
