Kato F, Nomura M, Nakamura K
Medicinal Research Laboratory, Central Research Institute, Kaisha, Japan.
Ann Rheum Dis. 1996 Aug;55(8):535-9. doi: 10.1136/ard.55.8.535.
To determine whether collagen induced arthritis (CIA) in mice can be satisfactorily induced by a single immunisation and whether this model has some advantages compared with conventional CIA, which is induced by two immunisations.
The incidence of arthritis was observed under different immunisation conditions (variation of species of Mycobacterium included in complete Freund's adjuvant and the method of emulsification) and immunological, histopathological, and pharmacological features were examined.
Under optimum immunisation conditions, joint inflammation developed two to three weeks after the primary immunisation with an incidence of 100% at four to five weeks. The progression of the arthritis was mild and was associated with moderate increases in concentrations of serum IgG against type II collagen. This CIA model was similar to the conventional model in histopathological and pharmacological features.
Murine CIA could be successfully induced by a single immunisation. An important feature of this model was a mild progression of joint inflammation. This feature seems to be of benefit for monitoring the development of arthritis from an early stage in the disease and for the development of novel antirheumatic drugs for such early stage patients.
确定单次免疫是否能成功诱导小鼠胶原诱导性关节炎（CIA），以及该模型与传统的两次免疫诱导的CIA模型相比是否具有某些优势。
观察不同免疫条件下（完全弗氏佐剂中包含的分枝杆菌种类变化及乳化方法）关节炎的发病率，并检测免疫学、组织病理学和药理学特征。
在最佳免疫条件下，初次免疫后两到三周出现关节炎症，四到五周时发病率达100%。关节炎进展轻微，与抗II型胶原血清IgG浓度适度升高有关。该CIA模型在组织病理学和药理学特征方面与传统模型相似。
单次免疫可成功诱导小鼠CIA。该模型的一个重要特征是关节炎症进展轻微。这一特征似乎有利于从疾病早期监测关节炎的发展，以及为这类早期患者开发新型抗风湿药物。
