Vitiello M V, Larsen L H, Moe K E, Borson S, Schwartz R S, Prinz P N
Department of Psychiatry and Behavioral Sciences, University of Washington, Seattle 98195-6560, USA.
Sleep. 1996 May;19(4):304-11. doi: 10.1093/sleep/19.4.304.
Elucidation of sleep-endocrine relationships requires frequent blood sampling during sleep recording. Unfortunately, such sampling can itself affect sleep and indirectly, hormonal patterns. We examined the effect of catheterization and frequent nighttime blood sampling on the sleep of a large sample of healthy older men and women. A total of 113 healthy older [69.1 +/- 0.6 years, mean +/- standard error of the mean (SEM) adults (68 women and 45 men) were studied. Following an adaptation night sleep was recorded during an undisturbed night and a night of periodic blood sampling via i.v. catheter. Lights out and lights on were significantly delayed and advanced, respectively, on the catheterization night, resulting in a significantly shorter time in bed (TIB). Total sleep time and sleep efficiency were significantly reduced, and sleep latency, total wake time and the number of awakenings from sleep of > or = 1 minute were significantly increased. Rapid eye movement (REM) sleep percentage of TIB was significantly reduced. Stages 3/4 sleep [slow wave sleep (SWS)] percentage of TIB was significantly reduced, as was total delta energy during SWS. With the exception of total sleep time and sleep latency, all sleep-wake and delta variables were significantly correlated between nights. This was particularly the case for SWS and the delta energy variables. When examined separately by gender, both men and women showed significant catheter-based sleep disturbance. However, SWS and delta energy measures in men were unaffected by catheterization. The data clearly demonstrate that both the sleep maintenance and sleep architecture of healthy older men and women are significantly impacted by nighttime blood sampling procedures. Of the various measures examined here, SWS measures appear to be the least disrupted, particularly in men. These findings need to be taken into account in any study examining sleep-endocrine relationships utilizing older subjects.
阐明睡眠与内分泌的关系需要在睡眠记录过程中频繁采集血样。不幸的是，这种采样本身会影响睡眠，并间接影响激素模式。我们研究了导管插入术和夜间频繁采血对大量健康老年男性和女性睡眠的影响。共研究了113名健康老年人[69.1±0.6岁，平均±平均标准误差（SEM）]，其中包括68名女性和45名男性。经过一个适应夜后，在一个不受干扰的夜晚以及通过静脉导管进行定期采血的夜晚记录睡眠情况。在导管插入术当晚，熄灯和开灯时间分别显著延迟和提前，导致卧床时间（TIB）显著缩短。总睡眠时间和睡眠效率显著降低，睡眠潜伏期、总觉醒时间以及睡眠中觉醒时间≥1分钟的次数显著增加。快速眼动（REM）睡眠占TIB的百分比显著降低。3/4期睡眠[慢波睡眠（SWS）]占TIB的百分比显著降低，SWS期间的总δ波能量也降低。除总睡眠时间和睡眠潜伏期外，所有睡眠-觉醒和δ波变量在两个夜晚之间均显著相关。SWS和δ波能量变量尤其如此。按性别分别检查时，男性和女性均表现出基于导管的显著睡眠障碍。然而，男性的SWS和δ波能量测量不受导管插入术影响。数据清楚地表明，夜间采血程序对健康老年男性和女性的睡眠维持和睡眠结构均有显著影响。在此处检查的各种测量指标中，SWS测量指标似乎受干扰最小，尤其是在男性中。在任何利用老年受试者研究睡眠与内分泌关系的研究中，都需要考虑这些发现。
