Bielański W, Konturek S J, Dobrzańska M J, Pytko-Polończyk J, Sito E, Marshall B J
Institute of Physiology, Jagiellonian University School of Medicine, Cracow, Poland.
J Physiol Pharmacol. 1996 Mar;47(1):91-100.
Etiologic role for Helicobacter pylori (Hp) seems to be well established in gastric pathology. The high urease activity of Hp can be used to detect this bacterium by non-invasive urea breath tests (UBT). We validated the microdose version of the test in which 37 kBq 14C-urea is given orally in capsule. With the cut off value > 100 DPM as positive, UBT results correlated highly significant with combined results for invasive methods i.e. CLOtest + histology score. The reproducibility of the test was 100%. The results obtained for the breath test performed locally were almost identical with that read at remote laboratory. The data found for fasting and fed states of subjects agreed in 87%. When 14C-urea was confined in the mouth of both Hp positive and Hp negative patients UBT showed the presence of urease activity in the mouth cavity. 14C-urea capsule based breath test is highly reliable, safe, and reproducible for detection of Hp in the stomach. Results can be obtained within 15 min if a scintilation counter is nearby, or breath samples can be mailed to a testing laboratory for analysis.
幽门螺杆菌（Hp）在胃部病理学中的病因学作用似乎已得到充分证实。Hp的高脲酶活性可用于通过非侵入性尿素呼气试验（UBT）检测这种细菌。我们验证了该试验的微剂量版本，即口服37 kBq的14C-尿素胶囊。以>100 DPM为阳性临界值，UBT结果与侵入性方法（即CLOtest + 组织学评分）的联合结果高度相关。该试验的重复性为100%。本地进行的呼气试验结果与远程实验室读取的结果几乎相同。受试者空腹和进食状态下的数据一致性为87%。当14C-尿素置于Hp阳性和Hp阴性患者口腔中时，UBT显示口腔中有脲酶活性。基于14C-尿素胶囊的呼气试验在检测胃内Hp方面高度可靠、安全且可重复。如果附近有闪烁计数器，15分钟内即可获得结果，或者呼气样本可邮寄至检测实验室进行分析。
