Huppertz H I, Mösbauer S, Busch D H, Karch H
Universitätskinderklinik, Würzburg, Germany.
Eur J Pediatr. 1996 Apr;155(4):297-302. doi: 10.1007/BF02002716.
To assess the contribution of the lymphocyte proliferation assay in response to borrelial antigens to establishing a diagnosis of Lyme arthritis (LA) the response to two strains of Borrelia burgdorferi was tested in peripheral blood lymphocytes of 103 children and adolescents with arthritis, among them 55 with LA and 48 control patients. Patients with LA had a significantly higher response to borrelial antigens than control patients. However, there were several patients with false positive and false negative test results. Specificity and sensitivity of the test were 78% and 77%. In patients with LA the test may turn positive after antibiotic therapy and remain positive for up to 19 months after the disappearance of arthritis. The test does not aid in prognosis or follow up. In one patient with seronegative LA specific lymphocyte proliferation and polymerase chain reaction for borrelial fla sequences in urine were positive.
Rarely the lymphocyte proliferation assay may aid in finding the correct diagnosis when clinical presentation and anti-borrelial serology do not match.
为评估淋巴细胞增殖试验对莱姆关节炎（LA）诊断的贡献，对103例患有关节炎的儿童和青少年外周血淋巴细胞中对两种伯氏疏螺旋体菌株的反应进行了检测，其中55例为LA患者，48例为对照患者。LA患者对伯氏疏螺旋体抗原的反应明显高于对照患者。然而，有几例患者的检测结果为假阳性和假阴性。该检测的特异性和敏感性分别为78%和77%。在LA患者中，抗生素治疗后该检测可能转为阳性，并在关节炎消失后长达19个月保持阳性。该检测无助于预后或随访。在1例血清阴性的LA患者中，尿液中伯氏疏螺旋体fla序列的特异性淋巴细胞增殖和聚合酶链反应呈阳性。
当临床表现和抗伯氏疏螺旋体血清学不匹配时，淋巴细胞增殖试验很少有助于做出正确诊断。
