Karch H, Huppertz H I, Böhme M, Schmidt H, Wiebecke D, Schwarzkopf A
Institut für Hygiene und Nikrobiologie, Universität Würzburg, Germany.
J Clin Microbiol. 1994 Sep;32(9):2312-4. doi: 10.1128/jcm.32.9.2312-2314.1994.
Since the possibility of asymptomatic infection with Borrelia burgdorferi has been suggested by a positive serology found in healthy subjects, we hypothesized that these subjects might excrete borrelial DNA sequences in urine as happens in patients with Lyme borreliosis. We found borrelial sequences by nested PCR in the urine samples from 3 of 13 healthy B. burgdorferi antibody-positive adults but not in urine samples from 79 antibody-negative healthy controls. After therapy with doxycycline, the urine samples were repeatedly negative for B. burgdorferi DNA. We conclude that urinary excretion of borrelial DNA sequences may occur in seropositive healthy subjects during asymptomatic infection. Demonstration of such sequences in urine must be interpreted cautiously and may not necessarily prove a borrelial cause of disease.
由于健康受试者血清学检测呈阳性提示存在无症状感染伯氏疏螺旋体的可能性，我们推测这些受试者可能会像莱姆病患者一样在尿液中排出伯氏疏螺旋体DNA序列。我们通过巢式PCR在13名伯氏疏螺旋体抗体阳性的健康成年人中的3人的尿液样本中发现了伯氏疏螺旋体序列，但在79名抗体阴性的健康对照者的尿液样本中未发现。用强力霉素治疗后，尿液样本中伯氏疏螺旋体DNA反复呈阴性。我们得出结论，在无症状感染期间，血清反应阳性的健康受试者可能会出现尿液中排出伯氏疏螺旋体DNA序列的情况。尿液中此类序列的检测结果必须谨慎解读，不一定能证明疾病是由伯氏疏螺旋体引起的。
