Murray P G, Niedobitek G, Kremmer E, Grässer F, Reynolds G M, Cruchley A, Williams D M, Müller-Lantzsch N, Young L S
Institute for Cancer Studies, University of Birmingham, U.K.
J Pathol. 1996 Jan;178(1):44-7. doi: 10.1002/(SICI)1096-9896(199601)178:1<44::AID-PATH471>3.0.CO;2-0.
A new monoclonal antibody has been used to examine immunohistochemically the expression of the Epstein-Barr virus (EBV)-encoded nuclear antigen (EBNA) 1 in virus-associated epithelial lesions. EBNA1 was detected in the tumour cell nuclei of 10/13 undifferentiated nasopharyngeal carcinomas and of 10/10 EBV-associated gastric carcinomas. EBNA1 was also detected in 13 of 16 oral hairy leukoplakia (HL) samples, where its expression was confined to nuclei in the upper epithelial cell layers whilst basal epithelial cells were negative. This observation is in agreement with previous studies demonstrating the absence of latent EBV infection in the basal cell compartment of HL and suggests an essential role for EBNA1, not only in latent EBV infection but also in virus replication.
一种新的单克隆抗体已被用于免疫组织化学检测爱泼斯坦-巴尔病毒（EBV）编码的核抗原（EBNA）1在病毒相关上皮病变中的表达。在13例未分化鼻咽癌中的10例以及10例EBV相关胃癌中的10例肿瘤细胞核中检测到EBNA1。在16例口腔毛状白斑（HL）样本中的13例中也检测到EBNA1，其表达局限于上皮细胞上层的细胞核，而基底上皮细胞为阴性。这一观察结果与先前的研究一致，先前研究表明HL的基底细胞区不存在潜伏性EBV感染，提示EBNA1不仅在潜伏性EBV感染中，而且在病毒复制中都起着重要作用。
