Okuda M, Hino K, Korenaga M, Yamaguchi Y, Katoh Y, Okita K
First Department of Internal Medicine, Yamaguchi University, School of Medicine, Yamaguchi, Japan.
Hepatology. 1999 Jan;29(1):217-22. doi: 10.1002/hep.510290117.
Hepatitis C virus (HCV) has been reported to potentially replicate in peripheral blood mononuclear cells (PBMCs), but direct information on the pathogenic implication of HCV infection in PBMCs is still limited. To investigate this issue, we compared the complexity of HCV quasispecies in serum, PBMCs, and livers of 13 patients with type C chronic liver disease. Hypervariable region 1 (HVR 1) was amplified by reverse-transcription polymerase chain reaction (RT-PCR), and the PCR products were subcloned and sequenced. Considerable differences in the complexity of HVR 1 quasispecies were found in the serum, PBMCs, and liver in all patients, and the predominant sequences from each source were mutually different in 3 (23%) patients. An amino acid sequence unique to each source existed as well as a sequence common to serum and PBMCs, common to serum and livers, or common to PBMCs and liver. These results suggest infection of PBMCs by HCV, and that HCV in PBMCs may be differently exposed to host immunity from that in liver.
据报道，丙型肝炎病毒（HCV）可能在外周血单核细胞（PBMCs）中复制，但关于HCV感染PBMCs的致病意义的直接信息仍然有限。为了研究这个问题，我们比较了13例丙型慢性肝病患者血清、PBMCs和肝脏中HCV准种的复杂性。通过逆转录聚合酶链反应（RT-PCR）扩增高变区1（HVR 1），并对PCR产物进行亚克隆和测序。在所有患者的血清、PBMCs和肝脏中，发现HVR 1准种的复杂性存在显著差异，并且在3例（23%）患者中，各来源的主要序列互不相同。每个来源都存在独特的氨基酸序列，以及血清和PBMCs共有的序列、血清和肝脏共有的序列或PBMCs和肝脏共有的序列。这些结果表明HCV感染了PBMCs，并且PBMCs中的HCV可能与肝脏中的HCV受到宿主免疫的方式不同。
