Mizoguchi N, Mizokami M, Orito E, Shibata H, Shibata H
Second Department of Medicine, Nagoya City University Medical School Kawasumi, Japan.
J Virol Methods. 1996 Apr 26;58(1-2):71-9. doi: 10.1016/0166-0934(95)01989-8.
Recently, Tsukiyama-Kohara et al. (1993) reported that most hepatitis C viruses (HCVs) in Japan can be classified into two types, type 1 and type 2, on the basis of the NS4 region nucleotide sequence. They developed a new assay in which antibodies against group-specific recombinant proteins of the NS4 region were measured by ELISA (serologically defined genotype, serotype). In the present study, we examined 306 patients with chronic liver disease due to HCV infection. The sensitivity of this assay was 98.7% (302/306). The serotype distribution of HCV was 230/306 (75.2%) for type 1, 65/306 (21.2%) for type 2, 7/306 (2.3%) for mixed, both being positive, and 4/306 (1.3%) indeterminate. The frequency of type 1 was significantly higher than that of type 2 (P < 0.01). There were no significant differences in clinical characteristics among the mixed and the indeterminate serotypes. Among the mixed-serotype patients, 4/7 (57.1%) showed seroconversion to a single serotype at 6 and 9 months later, although the serotypes of the indeterminate-serotype patients were also indeterminate at 6 and 9 months later. Using aliquots of the same serum samples, HCV genotyping was carried out by the reverse transcription polymerase chain reaction (RT-PCR) method using type-specific primers derived from the NS5 region of HCV to verify the specificity of this serotyping. The sensitivity of genotyping by RT-PCR was 167/183 (91.3%). The HCV genotypes determined by both methods were consistent in 161/183 (88.0%) of the cases, and there were no contradictory results for any sample between the two methods. These findings indicate that serological genotyping might be useful in determining the HCV genotype among Japanese patients with HCV infection.
最近，筑山小原等人（1993年）报告称，根据NS4区域核苷酸序列，日本的大多数丙型肝炎病毒（HCV）可分为1型和2型两种类型。他们开发了一种新的检测方法，通过酶联免疫吸附测定法（ELISA）检测针对NS4区域组特异性重组蛋白的抗体（血清学定义的基因型，血清型）。在本研究中，我们检查了306例因HCV感染导致慢性肝病的患者。该检测方法的灵敏度为98.7%（302/306）。HCV的血清型分布为：1型230/306（75.2%），2型65/306（21.2%），混合型（两者均为阳性）7/306（2.3%），不确定型4/306（1.3%）。1型的频率显著高于2型（P<0.01）。混合型和不确定型血清型之间的临床特征无显著差异。在混合型血清型患者中，4/7（57.1%）在6个月和9个月后出现血清转化为单一血清型，尽管不确定型血清型患者在6个月和9个月后的血清型也仍不确定。使用相同血清样本的等分试样，通过逆转录聚合酶链反应（RT-PCR）方法，使用源自HCV NS5区域的型特异性引物进行HCV基因分型，以验证这种血清分型的特异性。RT-PCR基因分型的灵敏度为167/183（91.3%）。两种方法确定的HCV基因型在161/183（88.0%）的病例中一致，两种方法对任何样本均未出现矛盾结果。这些发现表明，血清学分型可能有助于确定日本HCV感染患者的HCV基因型。
