基于连接酶链反应的检测方法与两种酶免疫测定法在检测非淋菌性尿道炎男性尿液标本中沙眼衣原体的性能比较。
Comparison among performances of a ligase chain reaction-based assay and two enzyme immunoassays in detecting Chlamydia trachomatis in urine specimens from men with nongonococcal urethritis.
作者信息
Deguchi T, Yasuda M, Uno M, Tada K, Iwata H, Komeda H, Maeda S, Latila V, Saito I, Kawada Y
机构信息
Department of Urology, Gifu University School of Medicine, Japan.
出版信息
J Clin Microbiol. 1996 Jul;34(7):1708-10. doi: 10.1128/JCM.34.7.1708-1710.1996.
We evaluated the performances of a ligase chain reaction (LCR)-based assay and two enzyme immunoassays (Chlamydiazyme and IDEIA) in the detection of Chlamydia trachomatis in urine specimens. We compared the results of testing urine specimens by these assays with those of urethral swab culture by examining samples from 131 men with nongonococcal urethritis. Discrepant results were analyzed by testing urethral swab specimens for C. trachomatis by a PCR-based assay. After the resolution of discrepant results, the sensitivity of urethral swab culture was 85.3%, whereas those of the LCR assay, Chlamydiazyme, and IDEIA with urine specimens were 94.1, 82.4, and 94.1%, respectively. The LCR assay and IDEIA were more sensitive than was urethral swab culture. In addition, the LCR assay, with a sensitivity equal to that of IDEIA, was more specific. Overall, the LCR assay proved to be superior to the enzyme immunoassays in detecting C. trachomatis in urine specimens. Testing urine specimens by LCR assay should be a helpful alternative method for diagnosing C. trachomatis urethral infection in men with nongonococcal urethritis.
我们评估了一种基于连接酶链反应（LCR）的检测方法以及两种酶免疫测定法（衣原体酶免疫测定法和IDEIA）在检测尿液标本中沙眼衣原体方面的性能。我们通过检测131例非淋菌性尿道炎男性的样本，将这些检测方法对尿液标本的检测结果与尿道拭子培养结果进行了比较。通过基于聚合酶链反应（PCR）的检测方法对尿道拭子标本进行沙眼衣原体检测，对不一致的结果进行分析。在解决不一致结果后，尿道拭子培养的敏感性为85.3%，而基于LCR的检测方法、衣原体酶免疫测定法以及使用尿液标本的IDEIA的敏感性分别为94.1%、82.4%和94.1%。LCR检测方法和IDEIA比尿道拭子培养更敏感。此外，LCR检测方法的敏感性与IDEIA相当，但特异性更高。总体而言，在检测尿液标本中的沙眼衣原体方面，LCR检测方法被证明优于酶免疫测定法。通过LCR检测方法检测尿液标本应该是诊断非淋菌性尿道炎男性沙眼衣原体尿道感染的一种有用的替代方法。
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