Bassiri M, Hu H Y, Domeika M A, Burczak J, Svensson L O, Lee H H, Mårdh P A
Institute of Clinical Bacteriology, University of Uppsala, Sweden.
J Clin Microbiol. 1995 Apr;33(4):898-900. doi: 10.1128/jcm.33.4.898-900.1995.
The performance of a plasmid-based ligase chain reaction (LCR) with urine specimens was compared with those of cell culture of cervical swabs and enzyme immunoassay with urine specimens for the detection of Chlamydia trachomatis infection in women who had attended a family planning clinic. The prevalence of chlamydial infection determined by LCR was 3.1%. Discrepant results among the three assays were resolved by testing urine by a second LCR assay based on the C. trachomatis chromosomal gene encoding the major outer membrane protein. Sensitivity, specificity, and positive and negative predictive values for the cell cultures were 56.3, 100, 100, and 98.4%, respectively, whereas those for the enzyme immunoassay were 18.8, 100, 100, and 97.1%, respectively, and those for LCR were 87.5, 100, 100, and 99.5%, respectively. LCR thus provides a highly sensitive and specific noninvasive screening method for detecting genital chlamydial infections in women.
在一家计划生育诊所就诊的女性中，对基于质粒的连接酶链反应（LCR）检测尿液标本沙眼衣原体感染的性能，与宫颈拭子细胞培养法及尿液标本酶免疫测定法进行了比较。通过LCR测定的衣原体感染患病率为3.1%。基于编码主要外膜蛋白的沙眼衣原体染色体基因的第二种LCR测定法检测尿液，解决了三种检测方法之间的差异结果。细胞培养法的敏感性、特异性、阳性预测值和阴性预测值分别为56.3%、100%、100%和98.4%，而酶免疫测定法的相应值分别为18.8%、100%、100%和97.1%，LCR的相应值分别为87.5%、100%、100%和99.5%。因此，LCR为检测女性生殖道衣原体感染提供了一种高度敏感且特异的非侵入性筛查方法。
