van Doornum G J, Buimer M, Prins M, Henquet C J, Coutinho R A, Plier P K, Tomazic-Allen S, Hu H, Lee H
Department of Public Health, Municipal Health Service of Amsterdam, The Netherlands.
J Clin Microbiol. 1995 Aug;33(8):2042-7. doi: 10.1128/jcm.33.8.2042-2047.1995.
The suitability of urine specimens from women and men for the detection of Chlamydia trachomatis infection by a ligase chain reaction (LCR)-based assay with plasmid primers was examined with a group of patients attending a sexually transmitted disease clinic in Amsterdam, The Netherlands. Cervical specimens from 15 of 237 (6.3%) women tested positive for C. trachomatis by cell culture. Of the 25 (10.5%) female urine samples that tested positive by the plasmid-LCR assay, 13 were obtained from cervical culture-positive women. Nine of the 12 plasmid-LCR-positive urine samples from cervical culture-negative women were confirmed to be positive by a second LCR assay with primers based on chromosomal DNA. Urethral specimens from 24 of 258 (9.3%) men were positive for C. trachomatis infection by cell culture. Of the 25 (9.7%) urine samples that tested positive by plasmid-LCR, 20 were from culture-positive men. All five of the LCR-positive urine samples from culture-negative men were confirmed to be positive by the LCR with chromosomal DNA primers. Relative to cell culture, testing by plasmid-LCR analysis of male urine samples had a sensitivity of 83.3% and a specificity of 97.9%; after resolution of discordant samples, these values were 86.2 and 100%, respectively. In the study with women, the sensitivities of plasmid-LCR analysis of cervical and urine specimens in comparison with cervical cell culture were 93.3 and 86.7%, respectively. After resolution of discrepant samples, the sensitivities of the plasmid-LCR test for cervical swabs and female urine samples were 96.3 and 92.6%, respectively. These results indicate that the plasmid-LCR-based assay is a very reliable, sensitive, convenient test for the detection of C. trachomatis infection in female and male urine specimens.
在荷兰阿姆斯特丹一家性传播疾病诊所就诊的一组患者中，研究了使用基于连接酶链反应（LCR）的质粒引物检测法检测男女尿液标本中沙眼衣原体感染的适用性。237名女性中的15名（6.3%）宫颈标本通过细胞培养检测出沙眼衣原体呈阳性。在通过质粒-LCR检测呈阳性的25份（10.5%）女性尿液样本中，有13份来自宫颈培养阳性的女性。来自宫颈培养阴性女性的12份质粒-LCR阳性尿液样本中的9份，通过基于染色体DNA的引物进行的第二次LCR检测被确认为阳性。258名男性中的24名（9.3%）尿道标本通过细胞培养检测出沙眼衣原体感染呈阳性。在通过质粒-LCR检测呈阳性的25份（9.7%）尿液样本中，有20份来自培养阳性的男性。来自培养阴性男性的所有5份LCR阳性尿液样本通过基于染色体DNA引物的LCR检测被确认为阳性。相对于细胞培养，对男性尿液样本进行质粒-LCR分析检测的灵敏度为83.3%，特异性为97.9%；在解决不一致样本后，这些值分别为86.2%和100%。在对女性的研究中，与宫颈细胞培养相比，对宫颈和尿液标本进行质粒-LCR分析的灵敏度分别为93.3%和86.7%。在解决不一致样本后，质粒-LCR检测对宫颈拭子和女性尿液样本的灵敏度分别为96.3%和92.6%。这些结果表明，基于质粒-LCR的检测法是一种非常可靠、灵敏且方便的检测男女尿液标本中沙眼衣原体感染的方法。
