Plotkin L I, Squiquera L, Mathov I, Galimberti R, Leoni J
IDEHU, Instituto de Estudios de la Inmunidad Humoral, CONICET-UBA, Buenos Aires, Argentina.
J Med Vet Mycol. 1996 Jan-Feb;34(1):43-8. doi: 10.1080/02681219680000071.
Enzymes capable of metabolizing lipids are essential for the growth of Malassezia furfur in vitro and in vivo. We designed a series of experiments to characterize the lipolytic system in this yeast. The optimal pH of the lipase system was 7.5 Lipase activity was detected in soluble and insoluble saline cell extracts and in supernatant from the cultures. Esterase activity screened in samples separated by native polyacrylamide gels showed that it was restricted to one band of low mobility. An FPLC analysis of the soluble saline extract demonstrated that the lipase activity was present in three major peaks with different protein composition as revealed by SDS-PAGE. The enzymatic activity and cell growth were first induced and later inhibited by increasing concentrations of polyethylene-sorbitan-monooleate (Tween-80). The characterization of the lipolytic system (e.g. its induction by substrate and the effect of pH and/or different cations) could help to explain the increment in the number of M. furfur infections related to alterations of surface lipids in the skin such as seborrheic dermatitis.
能够代谢脂质的酶对于糠秕马拉色菌在体外和体内的生长至关重要。我们设计了一系列实验来表征这种酵母中的脂解系统。脂肪酶系统的最适pH为7.5。在可溶性和不溶性盐细胞提取物以及培养物的上清液中检测到脂肪酶活性。在天然聚丙烯酰胺凝胶分离的样品中筛选的酯酶活性表明，它局限于一条低迁移率的条带。对可溶性盐提取物的FPLC分析表明，脂肪酶活性存在于三个主要峰中，SDS-PAGE显示其蛋白质组成不同。随着聚山梨醇酯-80（吐温-80）浓度的增加，酶活性和细胞生长首先被诱导，随后受到抑制。脂解系统的表征（例如其被底物诱导以及pH和/或不同阳离子的影响）有助于解释与皮肤表面脂质改变（如脂溢性皮炎）相关的糠秕马拉色菌感染数量的增加。
