Kappe R, Schulze-Berge A, Sonntag H G
Hygiene-Institut der Ruprecht-Karls-Universität, Heidelberg, Germany.
Mycoses. 1996 Jan-Feb;39(1-2):13-23. doi: 10.1111/j.1439-0507.1996.tb00078.x.
Eight Aspergillus antibody detection assays--three indirect haemagglutination assays (IHA-LD, IHA-Roche, IHA-Fumouze), three enzyme immunoassays (EIA-IgG, EIA-IgM, EIA-IgA, DDV) and two complement fixation tests (CF-metabolic and CF-somatic, Virion--and one latex agglutination test (LAT) for Aspergillus galactomannan antigen detection (Sanofi Pasteur) were evaluated in 14 patients with proven invasive aspergillosis (a total of 47 serum samples and one cerebrospinal fluid sample) and in 68 selected control individuals (one selected serum sample each). For the antibody tests, sensitivity ranged from 14% to 36% and specificity from 72% to 99%. The antigen detection test had a sensitivity of 36% and a specificity of 100%. Currently commercially available antibody detection assays for the serodiagnosis of invasive aspergillosis are inadequate. The antigen detection test appears to be highly specific, but lacks sufficient sensitivity.
在14例确诊为侵袭性曲霉病的患者（共47份血清样本和1份脑脊液样本）以及68名选定的对照个体（每人1份选定的血清样本）中，对8种曲霉抗体检测方法进行了评估，其中包括3种间接血凝试验（IHA-LD、IHA-罗氏、IHA-富穆兹）、3种酶免疫测定法（EIA-IgG、EIA-IgM、EIA-IgA、DDV）、2种补体结合试验（CF-代谢和CF-菌体，维里翁）以及1种用于曲霉半乳甘露聚糖抗原检测的乳胶凝集试验（LAT，赛诺菲巴斯德）。对于抗体检测，敏感性范围为14%至36%，特异性范围为72%至99%。抗原检测试验的敏感性为36%，特异性为100%。目前市售的用于侵袭性曲霉病血清诊断的抗体检测方法并不充分。抗原检测试验似乎具有高度特异性，但缺乏足够的敏感性。
