Moe H
Acta Pathol Microbiol Scand A. 1977 May;85(3):330-4.
The acute effect of vinblastine sulphate at a dosage of 0.2 mg per 100 g body weight on ameloblasts in the progress of differentiation in rat incisors, and the ability of these cells to develop into secretory ameloblasts after exposure to the drug, were studied under the light microscope. In the early stages of differentiation (exclusively dividing cells) no changes were seen. In more advanced stages of differentiation the cell apices were altered and the nuclei normally placed near the cell base were located at all levels in the ameloblast layer. These changes were reversible and the cells developed into secretory ameloblasts of normal appearance and function. Ameloblasts in the most advanced stage of differentiation in which secretion had begun were severely damaged, and the mature secretory ameloblast population derived from these cells was decimated and produced enamel matrix with abnormal structure. It is concluded that differentiating ameloblasts are relatively invulnerable to vinblastine, whereas in secretory ameloblasts the organelles directly engaged in the secretory processes are particularly exposed to damage from the drug.
研究了硫酸长春碱以每100克体重0.2毫克的剂量对大鼠切牙中处于分化进程的成釉细胞的急性作用，以及这些细胞在接触该药物后发育为分泌型成釉细胞的能力。在分化早期（仅为正在分裂的细胞）未观察到变化。在分化的更高级阶段，细胞顶端发生改变，通常位于细胞基部附近的细胞核在成釉细胞层的各个层面都有分布。这些变化是可逆的，细胞发育为外观和功能正常的分泌型成釉细胞。处于分化最晚期且已开始分泌的成釉细胞受到严重损伤，源自这些细胞的成熟分泌型成釉细胞群体大量减少，并产生结构异常的釉质基质。结论是，正在分化的成釉细胞对长春碱相对不敏感，而在分泌型成釉细胞中，直接参与分泌过程的细胞器特别容易受到该药物的损伤。
