Stichel C C, Kappler J, Junghans U, Koops A, Kresse H, Müller H W
Department of Neurology, University of Düsseldorf, Germany.
Brain Res. 1995 Dec 18;704(2):263-74. doi: 10.1016/0006-8993(95)01131-5.
Chondroitin sulfate proteoglycans are widespread extracellular matrix proteins and are specifically upregulated after CNS injury at the lesion site. Many proteoglycan core proteins have been described in the rat brain, but detailed analysis of individual proteoglycans expressed after injury are missing. The present study represents an initial attempt to assess the diversity and timing of lesion-induced expression of proteoglycans in order to elucidate their functional role in CNS injury and repair. Using immunocytochemical methods we analysed the expression of decorin and biglycan in the transected postcommissural fornix of the adult rat. Transection of the fornix induced the upregulation of both decorin and biglycan. However, their expression differed with respect to time course, regional extent and cellular localization. The rapid upregulation of decorin within a wide area around the lesion was followed by a massive appearance of biglycan that remained restricted to the transection site. Three months after lesion, differences of the area size of decorin- and biglycan-immunoreactivities were no longer detectable. Both proteoglycans were restricted to the lesion site and the fornix stumps. While decorin was primarily expressed by astrocytes, biglycan was deposited extracellularly in sheet-like structures. The upregulation of both proteoglycans persisted for at least up to 6 months after lesion. These strong but divergent lesion-induced expression patterns indicate important but different roles of decorin and biglycan in CNS injury.
硫酸软骨素蛋白聚糖是广泛存在的细胞外基质蛋白，在中枢神经系统损伤后，损伤部位会特异性上调表达。大鼠脑中已描述了许多蛋白聚糖核心蛋白，但对于损伤后表达的单个蛋白聚糖的详细分析尚付阙如。本研究初步尝试评估损伤诱导的蛋白聚糖表达的多样性和时间进程，以阐明它们在中枢神经系统损伤和修复中的功能作用。我们采用免疫细胞化学方法分析了成年大鼠切断的连合后穹窿中饰胶蛋白聚糖和双糖链蛋白聚糖的表达。穹窿切断术诱导了饰胶蛋白聚糖和双糖链蛋白聚糖的上调表达。然而，它们的表达在时间进程、区域范围和细胞定位方面存在差异。饰胶蛋白聚糖在损伤周围广泛区域迅速上调表达，随后双糖链蛋白聚糖大量出现，且仍局限于切断部位。损伤后三个月，饰胶蛋白聚糖和双糖链蛋白聚糖免疫反应性的面积差异不再可检测到。两种蛋白聚糖都局限于损伤部位和穹窿残端。饰胶蛋白聚糖主要由星形胶质细胞表达，而双糖链蛋白聚糖则以片状结构细胞外沉积。两种蛋白聚糖的上调表达在损伤后至少持续6个月。这些强烈但不同的损伤诱导表达模式表明饰胶蛋白聚糖和双糖链蛋白聚糖在中枢神经系统损伤中发挥着重要但不同的作用。
