Carbone A, Gloghini A, Vaccher E, Zagonel V, Pastore C, Dalla Palma P, Branz F, Saglio G, Volpe R, Tirelli U, Gaidano G
Division of Pathology, Centro di Riferimento Oncologico, Istituto Nazionale di Ricovero e Cura a Carattere Scientifico, Aviano, Italy.
Br J Haematol. 1996 Sep;94(3):533-43. doi: 10.1046/j.1365-2141.1996.d01-1826.x.
Primary effusions presenting as the sole lymphoma localization are also known as body-cavity-based-lymphoma (BCBL), and have been shown to carry Kaposi's sarcoma herpesvirus (KSHV) DNA sequences. The aim of this study was a comparative analysis of the clinical, pathologic and molecular features of BCBL and lymphomatous effusions secondary to tissue-based lymphomas occurring both in the general population and in HIV-1-infected individuals. All the lymphomatous effusion samples (seven AIDS-related and nine AIDS-unrelated) were subjected to an identical multiparameter investigation, including collection of clinical data, analysis of morphology and immunophenotype, as well as the study of viral sequences and genetic lesions. In six cases defined as BCBL (four AIDS-related and two AIDS-unrelated), the patients exhibited exclusive or predominant involvement of the body cavities. BCBL tended to display indeterminate phenotypes (4/6), whereas all AIDS-related and AIDS-unrelated lymphomatous effusions secondary to tissue-based lymphomas consistently expressed B-cell phenotype. Detection of KSHV DNA sequences was restricted to cases of BCBL (3/4 AIDS-related and 1/2 AIDS-unrelated), whereas EBV association (3/4) and expression of EBV-encoded antigens (LMP-1, 2/3; EBNA-2, 1/3) were confined to the AIDS-related BCBL. Overall, our results confirm that both AIDS-related and AIDS-unrelated BCBL preferentially associate with peculiar clinical, immunophenotypic and molecular features among lymphomatous effusions and therefore should be singled out as a specific clinico-pathologic entity.
仅表现为淋巴瘤定位的原发性积液也被称为体腔型淋巴瘤（BCBL），并且已被证明携带卡波西肉瘤疱疹病毒（KSHV）的DNA序列。本研究的目的是对普通人群和HIV-1感染个体中发生的BCBL与继发于组织型淋巴瘤的淋巴瘤性积液的临床、病理和分子特征进行比较分析。所有淋巴瘤性积液样本（7例与艾滋病相关，9例与艾滋病无关）均接受相同的多参数研究，包括临床数据收集、形态学和免疫表型分析，以及病毒序列和基因病变研究。在6例被定义为BCBL的病例（4例与艾滋病相关，2例与艾滋病无关）中，患者表现为体腔的排他性或主要受累。BCBL倾向于表现出不确定的表型（4/6），而所有继发于组织型淋巴瘤的与艾滋病相关和与艾滋病无关的淋巴瘤性积液均持续表达B细胞表型。KSHV DNA序列的检测仅限于BCBL病例（3/4与艾滋病相关，1/2与艾滋病无关），而EBV关联（3/4）和EBV编码抗原的表达（LMP-1，2/3；EBNA-2，1/3）仅限于与艾滋病相关的BCBL。总体而言，我们的结果证实，与艾滋病相关和与艾滋病无关的BCBL在淋巴瘤性积液中均优先与特殊的临床、免疫表型和分子特征相关，因此应被单独列为一种特定的临床病理实体。
