Otsuki T, Kumar S, Ensoli B, Kingma D W, Yano T, Stetler-Stevenson M, Jaffe E S, Raffeld M
Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
Leukemia. 1996 Aug;10(8):1358-62.
Herpesvirus-like DNA sequences have been identified in a high proportion of both AIDS-associated and classical Kaposi's sarcoma, and in a small percentage of AIDS-associated malignant lymphomas. To determine the extent of involvement of this new agent designated HHV-8 (human herpesvirus type 8) or KSHV (Kaposi's sarcoma-associated herpesvirus) in human malignant lymphomas, we analyzed 24 AIDS-associated lymphoid malignancies and 100 non-AIDS-associated lymphomas by PCR and Southern blot analysis. Three of 24 lymphoid malignancies from patients with AIDS demonstrated HHV-8 sequences by Southern blot and PCR analyses. The fourth was positive by PCR only. None of the non-AIDS-associated lymphomas contained HHV-8 sequences. All three Southern blot positive samples were derived from extranodal regions, two from pleural effusions, and one from a soft tissue mass in the thigh. This latter patient initially presented with a pleural effusion. The fourth PCR positive but Southern blot negative tumor was from a gingival lymphoma in a patient with a history of Kaposi's sarcoma. All tumors positive for HHV-8 were also positive for EBV. These results confirm a recent report that this novel herpesvirus may play a role in AIDS-associated lymphomas especially in those with body cavity presentation.
在相当比例的艾滋病相关型和经典型卡波西肉瘤中，以及一小部分艾滋病相关恶性淋巴瘤中，均已鉴定出类疱疹病毒DNA序列。为了确定这种新发现的被命名为HHV - 8（人类疱疹病毒8型）或KSHV（卡波西肉瘤相关疱疹病毒）的病原体在人类恶性淋巴瘤中的累及程度，我们通过聚合酶链反应（PCR）和Southern印迹分析，对24例艾滋病相关淋巴系统恶性肿瘤和100例非艾滋病相关淋巴瘤进行了分析。24例艾滋病患者的淋巴系统恶性肿瘤中，有3例经Southern印迹和PCR分析显示存在HHV - 8序列。第4例仅PCR呈阳性。所有非艾滋病相关淋巴瘤均未检测到HHV - 8序列。所有3例Southern印迹呈阳性的样本均来自结外区域，2例来自胸腔积液，1例来自大腿部软组织肿块。后一位患者最初表现为胸腔积液。第4例PCR阳性但Southern印迹阴性的肿瘤来自一名有卡波西肉瘤病史患者的牙龈淋巴瘤。所有HHV - 8阳性的肿瘤也均为EBV阳性。这些结果证实了最近的一项报告，即这种新型疱疹病毒可能在艾滋病相关淋巴瘤中起作用，尤其是在那些有体腔表现的淋巴瘤中。
