Petersen M, Sander L, Child R, van Onckelen H, Ulvskov P, Borkhardt B
Biotechnology Group, DIPS, Lyngby.
Plant Mol Biol. 1996 Jun;31(3):517-27. doi: 10.1007/BF00042225.
Seven distinct partial cDNAs, similar in sequence to previously described polygalacturonases (PGs), were amplified from cDNA derived from rape pod wall, dehiscence zone and leaves by the polymerase chain reaction. Northern analysis showed that one clone, PG35-8, was expressed at low levels in the dehiscence zone during the first five weeks after anthesis but was very abundantly expressed at week 6. In contrast, no PG35-8-related RNA was detected in the pod wall. Our data suggest that there are temporal and spatial correlations between the breakdown of the middle lamella, of the dehiscence zone cells and the pattern of synthesis of PG35-8 transcripts which may indicate a role for this particular PG in rape pod dehiscence. PG35-8 was used to isolate five cDNA clones from a rape dehiscence zone cDNA library. Restriction enzyme analysis and partial sequencing revealed that they were derived from four highly homologous transcripts which are probably allelic forms of a single gene. One full-length clone, RDPG1, was completely sequenced. The predicted protein of RDPG1 showed its highest identity with PG from apple fruit with an identity of 52%.
通过聚合酶链反应从油菜角果壁、开裂区和叶片的cDNA中扩增出7个不同的部分cDNA，其序列与先前描述的多聚半乳糖醛酸酶（PGs）相似。Northern分析表明，一个克隆PG35 - 8在花期后的前5周在开裂区低水平表达，但在第6周大量表达。相反，在角果壁中未检测到与PG35 - 8相关的RNA。我们的数据表明，中层细胞壁的分解、开裂区细胞的分解与PG35 - 8转录本的合成模式之间存在时间和空间相关性，这可能表明这种特定的PG在油菜角果开裂中起作用。利用PG35 - 8从油菜开裂区cDNA文库中分离出5个cDNA克隆。限制性酶切分析和部分测序表明，它们来自4个高度同源的转录本，可能是单个基因的等位形式。对一个全长克隆RDPG1进行了完全测序。RDPG1预测的蛋白质与苹果果实中的PG具有最高的同源性，同源性为52%。
